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Antibacterial, Antibiofilm, and Antiquorum Sensing Activities of Phytosynthesized Silver Nanoparticles Fabricated From Mespilus Germanica Extract Against Multidrug Resistance of Klebsiella Pneumoniae Clinical Strains Publisher Pubmed



Foroohimanjili F1 ; Mirzaie A2 ; Hamdi SMM1 ; Noorbazargan H3 ; Hedayati Ch M4 ; Dolatabadi A5 ; Rezaie H1 ; Bishak FM5
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Authors Affiliations
  1. 1. Department of Biology, Islamic Azad University, Central Tehran Branch, Tehran, Iran
  2. 2. Department of Biology, Islamic Azad University, Roudehen Branch, Roudehen, Iran
  3. 3. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  5. 5. Department of Biology, Islamic Azad University, Tehran North Branch, Tehran, Iran

Source: Journal of Basic Microbiology Published:2020


Abstract

The aim of the present work was to investigate the antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles (AgNPs) fabricated from Mespilus germanica extract against multidrug-resistant (MDR) Klebsiella pneumoniae strains. Fifty strains of K. pneumoniae were isolated from various clinical specimens. Biofilm-forming strains were identified using Congo red agar and polymerase chain reaction (PCR) techniques. Subsequently, the antibacterial activity of phytosynthesized AgNPs on MDR K. pneumoniae strains was investigated by broth microdilution assay and agar well-diffusion method. Finally (in the last step), the antibiofilm activity of phytosynthesized AgNPs was determined using microtiter plate assay and real-time PCR (RT-PCR) methods for the analysis of type 3 fimbriae (mrkA) and quorum-sensing system (luxS) gene expression. The results of this study showed that the phytosynthesized AgNPs had a spherical nanostructure with the mean size of 17.60 nm. The AgNPs exhibited dose-dependent antibacterial activity. The results of the microtiter plate and RT-PCR methods show that AgNPs inhibited the biofilm formation in MDR K. pneumoniae strains, and the expressions of mrkA and luxS genes were downregulated significantly in MDR strains after treatment with a subminimum inhibitory concentration of AgNPs. In conclusion, AgNPs effectively prevent the formation of biofilms and kill bacteria in established biofilms, which suggests that AgNPs might be a promising candidate for the prevention and treatment of biofilm-related infections caused by MDR K. pneumoniae strains. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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