The Effects of Oxygen–Ozone Therapy on Regulatory T-Cell Responses in Multiple Sclerosis Patients Publisher Pubmed



Tahmasebi S¹ ; Qasim MT² ; Krivenkova MV³ ; Zekiy AO⁴ ; Thangavelu L⁴ ; Aravindhan S⁵ ; Izadi M⁶ ; Jadidiniaragh F⁸ ; Ghaebi M⁷ ; Aslani S⁹ ; Aghebatmaleki L⁸ ; Ahmadi M⁷ ; Roshangar L⁷ Show Affiliations
Source: Cell Biology International Published:2021

Abstract

Multiple sclerosis (MS) is a common degenerative disorder of the central nervous system. The decreased frequency and dysfunction of Treg cells cause inflammation and disease progression. Ozone autohemotherapy can be used as a potential therapeutic approach to regulate the immune system responses and inflammation in MS. For this purpose, 20 relapsing-remitting multiple sclerosis patients were under treatment with ozone twice weekly for 6 months. The frequency of Treg cell, the expression levels of the Treg cell-related factors (FoxP3, IL-10, TGF-β, miR-17, miR-27, and miR-146A), and the secretion levels of IL-10 and TGF-β were assessed. We found a significant increase in the number of Treg cells, expression levels of FoxP3, miRNAs (miR-17 and miR-27), IL-10, and TGF-β factors in patients after oxygen–ozone (O2-O3) therapy compared to before treatment. In contrast, oxygen–ozone therapy notably decreased the expression level of miR-146a in treated patients. Interestingly, the secretion levels of both IL-10 and TGF-β cytokines were considerably increased in both serum and supernatant of cultured peripheral blood mononuclear cells in posttreatment condition compared to pretreatment condition. According to results, oxygen–ozone therapy raised the frequency of Treg cell and its relevant factors in treated MS patients. Oxygen–ozone therapy would contribute to improving the MS patients by elevating the Treg cell responses. © 2021 International Federation for Cell Biology