Effect of Herbal Cream Made by Frankincense (Boswellia Serrata Roxb. Ex Colebr.) and Yarrow (Achillea Millefolium L.) on the Incisional Wound Healing Model in Rats Publisher



Zargaran A ; Mirghazanfari S M ; Ziari K ; Soleymani S
Source: Journal of Medicinal Plants Published:2026

Abstract

Background: Boswellia serrata Roxb. ex Colebr and Achillea millefolium L. are two main plants cited in Persian medical manuscripts for wound healing. Objective: This animal study was planned to assess the effectiveness of a topical cream of B. serrata and Achillea millefolium propylene glycolic extract on the incisional wound healing model in Wistar rats. Methods: An incision wound was created on the dorsal part of 48 male Wistar rats (randomly divided into four groups of 12 each) after anesthesia with intra-peritoneal injection. Cream 5% (group 1), cream 10% (group 2), phenytoin (group 3) and placebo (group 4) were applied topically in each group for 14 days. Histopathological assessments and wound contraction percentage were documented on days 3, 7, 10, and 14. Furthermore, quality control and standardization of cream were performed. Results: Significant accelerated wound closure and increased re-epithelialization and fibroblasts was detected in the group 1 (cream 5%) compared with group 3 (phenytoin) and group 4 (placebo) on 7th day. The difference between two groups 1 and 2 was not significant. On the 10th of the experiment, all treated groups demonstrated significant wound closure in comparison to placebo group. Conclusion: Significant effects of topical semisolid formulation on improving wound could be incredibly promising for accelerating wound healing as potential natural remedy. However, future clinical studies are suggested to consider the precise effectiveness of this formulation in human. © 2023. Open access.