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Wound Healing Potential of Aqueous Extract of Elaeagnus Angustifolia Fruit With Down Regulation of Tnf-Alpha in Rat Publisher



Natanzi MM1 ; Khodaii Z1 ; Gerayeshnejad S2 ; Kamalinejad M3 ; Sharifi R2 ; Tavangar SM4 ; Dehpour AR5 ; Pasalar P2
Authors
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Authors Affiliations
  1. 1. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  2. 2. Department of biochemistry, School of Medicine, Tehran University of medical sciences, Tehran, Iran
  3. 3. Department of Pharmocognosy and Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Acta Medica Mediterranea Published:2017


Abstract

Wound healing properties of Elaeagnus angustifolia (EA) fruit has been used for a long time in traditional Iranian medicine. The present study was performed to investigate the biochemical mechanism of wound healing activity of this plant in rats. After creating full-thickness skin wounds on the back of the rats, they were randomly divided into three groups. Two groups as the treated and positive control received the extract of EA and mupirocin ointment, respectively and other group as the control group, was not subjected to any treatments. Isolated tissues from wounds were harvested for 5, 10, and 15 days and were then underwent biochemical analysis. Healing activity was assessed by the rate of wound contraction and collagen content. The in vivo antioxidant and anti-inflammatory activity was investigated to understand the mechanism of wound healing potency. The concentration levels of transforming growth factor beta 1 (TGF-β1) and tumor necrosis factor alpha (TNFα) were determined in wound tissues by an enzyme-linked immunosorbent assay (ELISA) and a real-time RT-PCR. The results indicated a significant increase in the percentage of wound contraction and collagen content in the treated group, when compared with the control and positive control groups. Both mRNA and protein levels of the TNFα were significantly reduced in the EA treated group. A significant decrease was also observed in Malondialdehyde and Myeloperoxidase content in the treated group compared to the control group. This study revealed that the EA extract accelerates cutaneous wound healing. This effect may confer its antioxidant and anti inflammatory activities. Therefore, EA extract may be useful in the current management of wound healing.ns.
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