Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Relationship Between Biofilm Formation and Mortality in Patients With Candida Tropicalis Candidemia Publisher Pubmed



Sasani E1 ; Khodavaisy S2 ; Rezaie S2 ; Salehi M3 ; Yadegari MH1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  2. 2. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Infectious Diseases and Tropical Medicine, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran

Source: Microbial Pathogenesis Published:2021


Abstract

Background: Biofilm formation by Candida species is an influential virulence factor in candidemia pathogenesis. We investigated the relationship between biofilm formation of Candida tropicalis isolates with the clinical characteristics and mortality outcomes in patients with candidemia. Materials and methods: Thirty-nine C. tropicalis isolates were recovered from patients with candidemia admitted to two university hospitals in Tehran, Iran. Biofilm mass and metabolic activity of C. tropicalis biofilms were assessed in vitro with two colorimetric methods. The sessile minimum inhibitory concentrations (SMICs) were evaluated in vitro by treating preformed biofilms with diluted concentrations of azoles according to CLSI-M27 A3/S4 protocol, followed by metabolic activity quantification. The expressions of ERG11, UPC2, MDR1, and CDR1 genes were also evaluated. Results: All C. tropicalis isolates produced biofilm. Respectively, higher <7-day and ≥7-day mortality rates were found among cases with high metabolic activity (46.7% vs. 13%, P = 0.03) and high biofilm mass (31.8% vs. 0, P = 0.029). Sessile cells had high resistance to fluconazole, voriconazole, and itraconazole. The azole minimum inhibitory concentrations (MICs) of C. tropicalis sessile were significantly greater than the planktonic minimum inhibitory concentrations (PMICs). In fluconazole-treated biofilms, the expression of ERG11 and UPC2 genes was increased. Conclusion: Our findings highlight the importance of C. tropicalis biofilm formation as an important factor in candidemia pathogenesis and the clinical outcome of patients with candidemia. © 2021 Elsevier Ltd
Related Docs
View other Related Docs
1. Virulence Factors and Azole-Resistant Mechanism of Candida Tropicalis Isolated From Candidemia, Mycopathologia (2021)
2. Evaluation of Virulence Factors and Azole Resistance Mechanisms of Candida Tropicalis Isolates From Head and Neck Cancer Patients With Opc, Iranian Journal of Microbiology (2023)
3. Epidemiology of Yeast Species Causing Bloodstream Infection in Tehran, Iran (2015-2017); Superiority of 21-Plex Pcr Over the Vitek 2 System for Yeast Identification, Journal of Medical Microbiology (2020)
4. Antifungal Susceptibility Pattern and Biofilm-Related Genes Expression in Planktonic and Biofilm Cells of Candida Parapsilosis Species Complex, Current Medical Mycology (2019)
5. Comparative in Vitro Activities of Seven Antifungal Drugs Against Clinical Isolates of Candida Parapsilosis Complex, Journal de Mycologie Medicale (2020)
6. Evaluation of Molecular Epidemiology, Clinical Characteristics, Antifungal Susceptibility Profiles, and Molecular Mechanisms of Antifungal Resistance of Iranian Candida Parapsilosis Species Complex Blood Isolates, Frontiers in Cellular and Infection Microbiology (2020)
7. Clinical, Epidemiological, and Mycological Features of Patients With Candidemia: Experience in Two Tertiary Referral Centers in Iran, Current Medical Mycology (2022)
8. Comparison of Biofilm-Producing Ability of Clinical Isolates of Candida Parapsilosis Species Complex, Journal de Mycologie Medicale (2019)
Experts (# of related papers)
View all Related Experts
Sadegh Khodavaisy (11)
Mohammadreza Salehi (8)
Other Related Docs
9. 21-Plex Pcr and Antifungal Susceptibility Aspects of Fungemia Agents in Pediatric Patients With Hematological Malignancies: A Single-Center Experience, Jundishapur Journal of Microbiology (2024)
10. Comparison of Pcr-Rflp With 21-Plex Pcr and Rdna Sequencing for Identification of Clinical Yeast Isolates, Mycopathologia (2021)
11. Oropharyngeal Candidiasis in Head and Neck Cancer Patients in Iran: Species Identification, Antifungal Susceptibility and Pathogenic Characterization, Journal de Mycologie Medicale (2018)
12. Anti-Biofilm Properties of Eucalyptol in Combination With Antifungals Against Candida Albicans Isolates in Patients With Hematological Malignancy, Archives of Microbiology (2022)
13. Effect of Voriconazole on Biofilm of Filamentous Species Isolated From Keratitis, Archives of Clinical Infectious Diseases (2021)
14. Synthesis, Cytotoxicity Evaluation, and Antifungal Activity of Novel Nitroglycerin Derivatives Against Clinical Candida Albi-Cans Isolates, Iranian Journal of Public Health (2021)
15. In Vitro Synergy of Echinocandins With Triazoles Against Fluconazole-Resistant Candida Parapsilosis Complex Isolates, Journal of Global Antimicrobial Resistance (2020)
16. Enhancement of the Anti-Biofilm Activity of Gold Nanoparticles-Itraconazole Conjugates in Resistant Candida Glabrata, Recent Advances in Anti-Infective Drug Discovery (2021)
17. M Eyerozyma Guilliermondii Species Complex: Review of Current Epidemiology, Antifungal Resistance, and Mechanisms, Brazilian Journal of Microbiology (2022)
18. Herpes Simplex Virus-1 Entrapped in Candida Albicans Biofilm Displays Decreased Sensitivity to Antivirals and Uva1 Laser Treatment, Annals of Clinical Microbiology and Antimicrobials (2017)
19. Multiplex Size Marker (Yeast Plex) for Rapid and Accurate Identification of Pathogenic Yeasts, Journal of Clinical Laboratory Analysis (2022)
20. Incidence and Spectrum of Yeast Species Isolated From the Oral Cavity of Iranian Patients Suffering From Hematological Malignancies, Journal of Oral Microbiology (2019)