Association Between a Pd-1 Gene Polymorphism and Antisperm Antibody-Related Infertility in Iranian Men Publisher Pubmed



Zamani MR¹ ; Asbagh FA² ; Massoud AH³ ; Salmaninejad A⁴ ; Massoud A¹ ; Rezaei N^{1, 5} Show Affiliations
Source: Journal of Assisted Reproduction and Genetics Published:2015

Abstract

Objective: Programmed cell death-1 (PD-1, Pdcd1), an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signalling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. This study investigates PD-1 gene polymorphism in patients with antisperm antibody-related infertility.; Methods: Genotyping was performed by polymerase chain reaction and restriction enzyme digestion (PCR-RFLP), this polymorphism was genotyped in 145 Iranian subjects (61 patients with antisperm antibody-related infertility and 84 healthy controls).; Results: Patients frequencies of the G/A genotype in comparison with healthy controls (38.2 % vs. 32.7 %, OR =1.21, P = 0.35) were not significantly different. However, G/G and A/A genotype frequencies between patients and healthy controls were significantly different (P = 0.042, P = 0.00001, respectively). Also, allele frequencies of this polymorphism were significantly different (P = 0.0012) in patients compared to healthy controls.; Conclusion: According to these results, there is a correlation between PD-1 gene polymorphism and susceptibility to antisperm antibody-related infertility in our study group. © 2014, Springer Science+Business Media New York.