Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats

Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats Publisher

Hassan FI¹ ; Didari T¹ ; Baeeri M¹ ; Gholami M² ; Haghiaminjan H³ ; Khalid M¹ ; Navaeinigjeh M¹ ; Rahimifard M¹ ; Solgi S¹ ; Abdollahi M^{1, 2} ; Mojtahedzadeh M^{1, 2}

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1. Pharmaceutical Sciences Research Center (PSRC), Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran
2. School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3. Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran

Source: Cell Journal Published:2020

Abstract

Objective: Metformin has a potent inhibitory activity against inflammation and oxidative stress, which inevitably occur in sepsis-associated encephalopathy (SAE). The precise mechanisms underlying neuroprotective effects of metformin in SAE, are still unclear. In the present work, the protective effect of metformin on SAE using cecal ligation and puncture (CLP) model of sepsis, was assessed. Materials and Methods: In this experimental study, CLP procedure was performed in Wistar rats and 50 mg/kg metformin was administered immediately. Specific markers of sepsis severity, inflammation, blood brain barrier (BBB) dysfunction, and brain injury, were investigated. Specific assay kits and real-time polymerase chain reaction (RT-PCR) were used. Histopathological assessment was also carried out. Results: Treatment with metformin decreased murine sepsis score (MSS), lactate, platelet lymphocyte ratio (PLR), and high mobility group box (HMGB1) levels. The expression levels of claudin 3 (Cldn3) and claudin 5 (Cldn5) were increased following treatment with metformin. Metformin decreased the expression of S100b, neuron specific enolase (Nse), and glial fibrillary acidic protein (Gfap). Conclusion: Our study suggests that metformin may inhibit inflammation and increase tight junction protein expressions which may improve BBB function and attenuate CLP-induced brain injury. Hence, the potential beneficial effects of metformin in sepsis, should be considered in future. © 2020 Royan Institute (ACECR). All rights reserved.

2. Short-Term Effects of Metformin on Cardiac and Peripheral Blood Cells Following Cecal Ligation and Puncture-Induced Sepsis, Drug Research (2021)

3. Metformin Accelerates Myelin Recovery and Ameliorates Behavioral Deficits in the Animal Model of Multiple Sclerosis Via Adjustment of Ampk/Nrf2/Mtor Signaling and Maintenance of Endogenous Oligodendrogenesis During Brain Self-Repairing Period, Pharmacological Reports (2020)

4. Neuroprotective Effects of Metformin Against Aβ-Mediated Inhibition of Long-Term Potentiation in Rats Fed a High-Fat Diet, Brain Research Bulletin (2016)

5. Evaluation of Metformin Effects in the Chronic Phase of Spontaneous Seizures in Pilocarpine Model of Temporal Lobe Epilepsy, Metabolic Brain Disease (2018)

6. Effect of Metformin on Epidermal Neural Crest Stem Cells and Their Potential Application in Ameliorating Paclitaxel-Induced Neurotoxicity Phenotype, Stem Cell Reviews and Reports (2024)

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Mojtaba Mojtahedzadeh (2)

Mohammad Abdollahi (2)

Style	Citing Format
MLA	Hassan FI, et al.. "Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats." Cell Journal, vol. 22, no. , 2020, pp. 29-37.
APA	Hassan FI, Didari T, Baeeri M, Gholami M, Haghiaminjan H, Khalid M, Navaeinigjeh M, Rahimifard M, Solgi S, Abdollahi M, Mojtahedzadeh M (2020). Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats. Cell Journal, 22(), 29-37.
Chicago	Hassan FI, Didari T, Baeeri M, Gholami M, Haghiaminjan H, Khalid M, Navaeinigjeh M, et al.. "Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats." Cell Journal 22, no. (2020): 29-37.
Harvard	Hassan FI et al. (2020) 'Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats', Cell Journal, 22(), pp. 29-37.
Vancouver	Hassan FI, Didari T, Baeeri M, Gholami M, Haghiaminjan H, Khalid M, et al.. Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats. Cell Journal. 2020;22():29-37.
BibTex	@article{ author = {Hassan FI and Didari T and Baeeri M and Gholami M and Haghiaminjan H and Khalid M and Navaeinigjeh M and Rahimifard M and Solgi S and Abdollahi M and Mojtahedzadeh M}, title = {Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats}, journal = {Cell Journal}, volume = {22}, number = {}, pages = {29-37}, year = {2020} }
RIS	TY - JOUR AU - Hassan FI AU - Didari T AU - Baeeri M AU - Gholami M AU - Haghiaminjan H AU - Khalid M AU - Navaeinigjeh M AU - Rahimifard M AU - Solgi S AU - Abdollahi M AU - Mojtahedzadeh M TI - Metformin Attenuates Brain Injury by Inhibiting Inflammation and Regulating Tight Junction Proteins in Septic Rats JO - Cell Journal VL - 22 IS - SP - 29 EP - 37 PY - 2020 ER -

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