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Three Liquid Phase Membrane Extraction Method Based on the Electro-Migration Assisted by High-Performance Liquid Chromatography for the Analysis of Carcinogenic Heterocyclic Aromatic Amines in Steak Samples Publisher Pubmed



Barzegar F1 ; Nabizadeh S1 ; Kamankesh M2, 3 ; Mohammadi A1, 4 ; Shariatifar N5
Authors
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Authors Affiliations
  1. 1. Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Food Safety Research Center (salt), Semnan University of Medical Sciences, Semnan, Iran
  3. 3. School of Pharmacy, Semnan University of Medical Sciences, Semnan, Iran
  4. 4. Food Safety Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Food Chemistry Published:2025


Abstract

A combination of microwave digestion and three-phase hollow fiber electromembrane extraction (HF-EME) with high-performance liquid chromatography (HPLC) was designed to determine carcinogenic heterocyclic aromatic amines (HAAs) in steak samples. The recorded fingers of merit (LOD: 1.4–1.6 ng/g, LOQ: 4.5–5.2 ng/g, RSD: 6.1–7.2 %, recovery: 91–95 % and EF: 107–112) underlines the adequacy of the proposed technique. The total of 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), 2-amino-3,8-dimethylimidazo [4,5-f] quinoxaline (MeIQx), 2-amino-3,4 dimethylimidazo[4,5-f] quinoline (MeIQ) and 2-amino-1-methyl-6-phenylimidazo[4′5-b] pyridine (PhIP) in steak samples were 93.46 ± 4.93–174.52 ± 10.91 ng/g, 31.65 ± 1.87–877.97 ± 56.19 ng/g, 10.08 ± 1.01–535.73 ± 18.93 ng/g, and 62.56 ± 1.89–341.23 ± 41.45 ng/g, respectively. Lamb steak contain highest amount of MeIQx (877.97 ± 56.19 ng/g) and beef steak showed lowest concentration of MeIQ (10.08 ± 1.07 ng/g). In conclusion, several interesting features such as great recovery, suitable enrichment factor, very low sample amount and organic solvent requirement and short extraction time make this novel method appropriate for the analysis of the negligible levels of HAAs in complex food matrices. © 2024 Elsevier Ltd
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