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Efficacy of Dental Pulp-Derived Stem Cells Conditioned Medium Loaded in Collagen Hydrogel in Spinal Cord Injury in Rats: Stereological Evidence Publisher Pubmed



Asadigolshan R1 ; Razban V2 ; Mirzaei E3 ; Rahmanian A4 ; Khajeh S5 ; Mostafavipour Z6 ; Dehghani F7
Authors
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Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
  3. 3. Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Department of Neurosurgery, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Bone and Joint Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  6. 6. Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  7. 7. Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Journal of Chemical Neuroanatomy Published:2021


Abstract

Spinal cord injury (SCI) causes histological alterations which in turn affects functional activity. Studies have demonstrated that dental pulp-derived stem cells conditioned medium has beneficial effects on the nervous system. Besides, collagen hydrogel acts as a drug releasing system in SCI investigations. This research aimed to evaluate effects of dental pulp-derived stem cells conditioned medium loaded in collagen hydrogel in SCI. After culturing of Stem cells from human exfoliated deciduous teeth (SHEDs), SHED-conditioned medium (SHED-CM) was harvested and concentrated. Collagen hydrogel containing SHED-CM was prepared. The rats were divided into five groups receiving laminectomy, compressive SCI with or without intraspinal injection of biomaterials (SHED-CM and collagen hydrogel with or without SHED-CM). After 6 weeks, histological parameters were estimated using stereological methods. The total volume of preserved white matter and gray matter (p < 0.05) as well as the total number of neurons and oligodendrocytes in the rats received SHED-CM loaded in collagen hydrogel were significantly higher, and also lesion volume and lesion length were significantly lower (p < 0.05) compared to those of the other injured groups. In conclusion, intraspinal administration of SHED-CM loaded in collagen hydrogel leads to neuroprotection, proposing a cell-free therapeutic approach in SCI. © 2021