Evaluation of Differentiation Quality of Several Differentiation Inducers of Bone Marrow-Derived Mesenchymal Stem Cells to Nerve Cells by As-Sessing Expression of Beta-Tubulin 3 Marker: A Systematic Review Publisher Pubmed



Fath MK¹ ; Zahedi F² ; Hashemi ZS³ ; Khalili S^{4, 5} Show Affiliations
Source: Current Stem Cell Research and Therapy Published:2021

Abstract

Neurological diseases have different etiological causes. Contemporary, developing an ef-fective treatment for these diseases is an ongoing challenge. Cell therapy is recognized as one of the promising solutions for the treatment of these diseases. Amongst various types of stem cells, bone marrow-derived mesenchymal stem cells (BM-MSC) are known to be the most widely used stem cells. These cells are endowed with appealing properties such as the ability to differentiate into other cell types, including the muscle, liver, glial, and nerve cells. In this review study, we have systematically evaluated the ability of a variety of chemical compounds used in the last ten years to differentiate BM-MSCs into neurons by examining the expression level of beta-tubulin 3 protein. The present study is a systematic search performed at three separate databases, including PubMed, ScienceDirect, and Embase from August 2009 to August 2019. The search results in the three men-tioned databases were 323 articles and finally, 8 articles were selected and carefully examined con-sidering the inclusion and exclusion criteria. The results showed that different chemical compounds such as ROCK inhibitors, sex steroid hormones, bFGF, NGF, Noggin, 4 OHT, TSA, VPA, Antidepressants, Neurosteroids (Dex and E2), and DHA are involved in different signaling path-ways, such as ERK, AKT, BMP, DHA / GPR40, Rho-dependent phosphorylation, and histone deacetylase inhibitors. Further investigation of these signaling pathways may open the way for bet-ter differentiation of BM-MSCs into neurons. © 2021 Bentham Science Publishers.