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Novel Delivery of Sorafenib by Natural Killer Cell-Derived Exosomes-Enhanced Apoptosis in Triple-Negative Breast Cancer Publisher Pubmed



Hashemi ZS1 ; Ghavami M2 ; Kiaie SH3, 4 ; Mohammadi F5 ; Barough MS1 ; Khalili S6 ; Hosseinifarjam Z1 ; Mossahebimohammadi M7 ; Sheidary A8 ; Ghavamzadeh A9 ; Forooshani RS1
Authors
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Authors Affiliations
  1. 1. Atmp Department, Breast Cancer Research Center, Motamed Cancer Institute, Acecr, Tehran, 15179/64311, Iran
  2. 2. Pathology Department, Dalhousie University, Halifax, B3H 4R2, Canada
  3. 3. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran
  4. 4. Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, 6715847141, Iran
  5. 5. Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, 1417653761, Iran
  6. 6. Department of Biology Sciences, Shahid Rajaee Teacher Training University, Tehran, 167815811, Iran
  7. 7. School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325027, China
  8. 8. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417653761, Iran
  9. 9. Cancer & Cell Therapy Research Center, Tehran University of Medical Sciences, Tehran, 1417653761, Iran

Source: Nanomedicine Published:2023


Abstract

Aim: We investigated the delivery of sorafenib (SFB) to breast cancer spheroids by natural killer cell-derived exosomes (NK-Exos). Methods: SFB-NK-Exos were constructed by electroporation. Their antitumor effects were evaluated by methyl thiazolyl tetrazolium, acridine orange/ethidium bromide, 4′,6-diamidino-2-phenylindole, annexin/propidium iodide, scratch and migration assay, colony formation, RT-PCR, western blot and lipophagy tests. Result: The loading efficacy was 46.66%. SFB-NK-Exos-treated spheroids showed higher cytotoxic effects (33%) and apoptotic population (44.9%). Despite the reduction of SFB concentration in the SFB-NK-Exos formulation, similar cytotoxic effects to those of free SFB were observed. Increased intracellular trafficking, sustained release of the drug and selective inhibitory effects demonstrated efficient navigation. Conclusion: This is the first report for SFB loading into NK-Exos, which led to significant cytotoxic intensification against cancer cells. © 2023 Future Medicine Ltd.
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