Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly

Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly Publisher Pubmed

Zand E¹ ; Rajablou E^{1, 2} ; Siadat SF³ ; Beiki B⁴ ; Akbarinejad V⁵ ; Amorim CA⁶ ; Valojerdi MR⁷ ; Tahaei LA¹ ; Fathi R¹

Show Affiliations

1. Department of Embryology, Royan Institute for Reproductive Biomedicine, Reproductive Biomedicine Research Center, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
2. Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
3. Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
4. Skin and Stem Cell Research Center, Tehran University of Medical Science, Tehran, Iran
5. Faculty of Veterinary Medicine, Department of Theriogenology, University of Tehran, Tehran, Iran
6. Institut de Recherche Experimentale et Clinique, Pole de Recherche en Physiopathologie de la Reproduction, Universite Catholique de Louvain, Brussels, Belgium
7. Faculty of Medical Sciences, Department of Anatomy, Tarbiat Modares University, Tehran, Iran

Source: PLoS ONE Published:2023

Abstract

Main objective Due to Human Wharton’s Jelly (HWJ) could be applied in tissue engineering as a bio scaffold, the present study was conducted to investigate the effects of HWJ hydrogel on in vitro culture and auto-transplantation of mouse ovarian follicles. Materials and methods HWJ was isolated from umbilical cord and decellularized with SDS/Tris/EDTA. DNA, Collagen and Glycosaminoglycans (GAGs) were measured. Decellularized Wharton’s Jelly (DWJ) was dissolved to make Wharton’s Jelly Hydrogel (WJH), and composited with Alginate (ALG) (1.5%) in equal ratio (WJH+ALG). Then, mouse preantral follicles were isolated and encapsulated in 10μL droplets of WJH and randomly considered for both 14 days culture and auto-transplantation. Results Collagen, GAGs and DNA evaluations showed majority of WJ cells have been removed and MTT approved no toxicity. Degradation rate and rheological analysis represented optimal hydrogel compatibility. The data from in vitro culture revealed significant antral formation in WJH+ALG (P<0.05). In transplantation, follicles failed to survive in ALG; however, survived in WJH+ALG to antral stage (P<0.05). VEGF and CD34 had greater expression in WJH +ALG than ALG (P< 0.05). Conclusion Wharton’s jelly hydrogel and Alginate compound is interesting composite for successful development of mouse preantral follicles in both 3D in vitro culture and transplantation. Copyright: © 2023 Zand et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Style	Citing Format
MLA	Zand E, et al.. "Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly." PLoS ONE, vol. 18, no. 9 September, 2023, pp. -.
APA	Zand E, Rajablou E, Siadat SF, Beiki B, Akbarinejad V, Amorim CA, Valojerdi MR, Tahaei LA, Fathi R (2023). Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly. PLoS ONE, 18(9 September), -.
Chicago	Zand E, Rajablou E, Siadat SF, Beiki B, Akbarinejad V, Amorim CA, Valojerdi MR, Tahaei LA, Fathi R. "Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly." PLoS ONE 18, no. 9 September (2023): -.
Harvard	Zand E et al. (2023) 'Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly', PLoS ONE, 18(9 September), pp. -.
Vancouver	Zand E, Rajablou E, Siadat SF, Beiki B, Akbarinejad V, Amorim CA, et al.. Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly. PLoS ONE. 2023;18(9 September):-.
BibTex	@article{ author = {Zand E and Rajablou E and Siadat SF and Beiki B and Akbarinejad V and Amorim CA and Valojerdi MR and Tahaei LA and Fathi R}, title = {Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly}, journal = {PLoS ONE}, volume = {18}, number = {9 September}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Zand E AU - Rajablou E AU - Siadat SF AU - Beiki B AU - Akbarinejad V AU - Amorim CA AU - Valojerdi MR AU - Tahaei LA AU - Fathi R TI - Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton’S Jelly JO - PLoS ONE VL - 18 IS - 9 September SP - EP - PY - 2023 ER -

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