Effective Attenuation of Vascular Restenosis Following Local Delivery of Chitosan Decorated Sirolimus Liposomes Publisher Pubmed



Haeri A¹ ; Sadeghian S² ; Rabbani S² ; Anvari MS² ; Ghassemi S¹ ; Radfar F² ; Dadashzadeh S^{1, 3} Show Affiliations
Source: Carbohydrate Polymers Published:2017

Abstract

Assuring the efficient local delivery via biocompatible nanosystems can be considered as a promising therapy for restenosis. The aim of the present study was preparation, in vitro characterization, and in vivo efficacy evaluation of sirolimus containing chitosan decorated liposomes for restenosis treatment. Liposomes were coated with chitosan, leading to ∼38 nm increase in the particle size and a positive shift in the zeta potential from −1 mV to +21 mV. Chitosan modification was also confirmed by TEM, increased stability against detergent solubilization, and FTIR analyses. High entrapment efficiency (≥83%) and sustained release behaviors were demonstrated in both coated and uncoated vesicles. Compared to control groups, treatment of balloon injured rats with uncoated and chitosan-coated liposomes (50 μg sirolimus) significantly reduced stenosis by 39% and 62%, respectively. The effect was also confirmed by immunohistochemical and in vivo CT angiography imaging studies. Chitosan-coated liposomes could be a novel platform for restenosis treatment. © 2016 Elsevier Ltd