Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells

Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells Publisher Pubmed

Salehi M^{1, 2} ; Ardeshirylajimi A³ ; Mossahebimohammadi M³ ; Kondori Z¹ ; Jorjani M⁴

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1. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Departmet of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Department of Stem Cell Biology, Stem Cell Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Neurosience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Cellular and Molecular Biology Published:2015

Abstract

Central nervous system (CNS) injuries cause to variable disorders in people around the world without any decisive treatment. Use of embryonic stem cells (ESCs) would be helpful in repairing of neural system damages. Somatic cell nuclear transfer (SCNT) is a way for implanting ESCs with lowest possible rejection. In the present study, mouse nuclear transfers ESCs (ntESCs) ability in differentiation to oligodendrocyte progenitor cells (OPCs) was investigated by morphological study, RT-PCR and flow cytometry analysis. Bi-polar and tri-polar, OPCs were observed in stem cells cultured in differentiation medium after four weeks. Result of gene expression analysis demonstrated that differentiated stem cells were expressed most important OPCs related gene markers after differentiation period. Moreover, flow cytometry analysis carried out to confirm other results that showed differentiated stem cells significantly expressed NG2 and O4 as critical OPC surface markers. Taken together, it seems that mouse ntESCs showed highly potential for OPC differentiation and could be an appropriate candidate for stem cell therapies. © 2015.

2. Efficacy of Dental Pulp-Derived Stem Cells Conditioned Medium Loaded in Collagen Hydrogel in Spinal Cord Injury in Rats: Stereological Evidence, Journal of Chemical Neuroanatomy (2021)

3. Neural Stem/Progenitor Cell Transplantation for Spinal Cord Injury Treatment; a Systematic Review and Meta-Analysis, Neuroscience (2016)

4. Advanced Approaches to Regenerate Spinal Cord Injury: The Development of Cell and Tissue Engineering Therapy and Combinational Treatments, Biomedicine and Pharmacotherapy (2022)

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Style	Citing Format
MLA	Salehi M, et al.. "Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells." Cellular and Molecular Biology, vol. 61, no. 2, 2015, pp. 56-59.
APA	Salehi M, Ardeshirylajimi A, Mossahebimohammadi M, Kondori Z, Jorjani M (2015). Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells. Cellular and Molecular Biology, 61(2), 56-59.
Chicago	Salehi M, Ardeshirylajimi A, Mossahebimohammadi M, Kondori Z, Jorjani M. "Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells." Cellular and Molecular Biology 61, no. 2 (2015): 56-59.
Harvard	Salehi M et al. (2015) 'Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells', Cellular and Molecular Biology, 61(2), pp. 56-59.
Vancouver	Salehi M, Ardeshirylajimi A, Mossahebimohammadi M, Kondori Z, Jorjani M. Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells. Cellular and Molecular Biology. 2015;61(2):56-59.
BibTex	@article{ author = {Salehi M and Ardeshirylajimi A and Mossahebimohammadi M and Kondori Z and Jorjani M}, title = {Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells}, journal = {Cellular and Molecular Biology}, volume = {61}, number = {2}, pages = {56-59}, year = {2015} }
RIS	TY - JOUR AU - Salehi M AU - Ardeshirylajimi A AU - Mossahebimohammadi M AU - Kondori Z AU - Jorjani M TI - Oligodendrocyte Progenitor Cells Differentiation of Nuclear Transferred Mouse Embryonic Stem Cells JO - Cellular and Molecular Biology VL - 61 IS - 2 SP - 56 EP - 59 PY - 2015 ER -

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