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Blockade of Vascular Endothelial Growth Factor Receptors by Tivozanib Has Potential Anti-Tumour Effects on Human Glioblastoma Cells Publisher Pubmed



Momeny M1 ; Moghaddaskho F1 ; Gortany NK2 ; Yousefi H3 ; Sabourinejad Z4 ; Zarrinrad G1 ; Mirshahvaladi S5 ; Eyvani H1 ; Barghi F1 ; Ahmadinia L1 ; Ghazikhansari M2 ; Dehpour AR2 ; Amanpour S6 ; Tavangar SM4 Show All Authors
Authors
  1. Momeny M1
  2. Moghaddaskho F1
  3. Gortany NK2
  4. Yousefi H3
  5. Sabourinejad Z4
  6. Zarrinrad G1
  7. Mirshahvaladi S5
  8. Eyvani H1
  9. Barghi F1
  10. Ahmadinia L1
  11. Ghazikhansari M2
  12. Dehpour AR2
  13. Amanpour S6
  14. Tavangar SM4
  15. Dardaei L7
  16. Emami AH8
  17. Alimoghaddam K1
  18. Ghavamzadeh A1
  19. Ghaffari SH1
Show Affiliations
Authors Affiliations
  1. 1. Haematology/Oncology and Stem Cell Transplantation Research Centre, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pharmacology, School of Medicine Tehran, University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Deparmtent of Pathology, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Molecular Systems Biology, Cell Science Research Centre, Royan Institute for Stem Cell Biology and Technology, Tehran, Iran
  6. 6. Cancer Biology Research Centre, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Medicine, Harvard Medical School, Boston, MA, United States
  8. 8. Division of Oncology, Department of Internal Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Scientific Reports Published:2017


Abstract

Glioblastoma (GBM) remains one of the most fatal human malignancies due to its high angiogenic and infiltrative capacities. Even with optimal therapy including surgery, radiotherapy and temozolomide, it is essentially incurable. GBM is among the most neovascularised neoplasms and its malignant progression associates with striking neovascularisation, evidenced by vasoproliferation and endothelial cell hyperplasia. Targeting the pro-angiogenic pathways is therefore a promising anti-glioma strategy. Here we show that tivozanib, a pan-inhibitor of vascular endothelial growth factor (VEGF) receptors, inhibited proliferation of GBM cells through a G2/M cell cycle arrest via inhibition of polo-like kinase 1 (PLK1) signalling pathway and down-modulation of Aurora kinases A and B, cyclin B1 and CDC25C. Moreover, tivozanib decreased adhesive potential of these cells through reduction of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Tivozanib diminished GBM cell invasion through impairing the proteolytic cascade of cathepsin B/urokinase-type plasminogen activator (uPA)/matrix metalloproteinase-2 (MMP-2). Combination of tivozanib with EGFR small molecule inhibitor gefitinib synergistically increased sensitivity to gefitinib. Altogether, these findings suggest that VEGFR blockade by tivozanib has potential anti-glioma effects in vitro. Further in vivo studies are warranted to explore the anti-tumour activity of tivozanib in combinatorial approaches in GBM.
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