The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies

The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies Publisher

Nasab AS ; Dalband AM ; Mohammadzadeh I ; Lajevardi Z ; Moafi M ; Ebrahimi MJ ; Nourirad SN ; Asadi A ; Efazati A ; Beirami A ; Hasanzadeh M ; Nikoohemmat M ; Zeidabadi AA ; Alinezhad A Show All Authors

Nasab AS
Dalband AM
Mohammadzadeh I
Lajevardi Z
Moafi M
Ebrahimi MJ
Nourirad SN
Asadi A
Efazati A
Beirami A
Hasanzadeh M
Nikoohemmat M
Zeidabadi AA
Alinezhad A
Zolfaghari F
Hajibeygi R
Vakili K
Bayat AH
Parvardeh S
Farahani RM
Sarkala HB
Sani M
Moghaddam MH
Aliaghaei A

Source: 3 Biotech Published:2026

Abstract

Tramadol has been classified as a prohibited substance in several countries due to a significant rise in overdoses and related deaths over the past decade. Black barberry (BB) has demonstrated therapeutic potential in various conditions, including metabolic, neurodegenerative, and cardiovascular disorders. This study investigated the protective effects of BB against tramadol-induced cognitive and neural impairments. Thirty-six rats were divided into three groups: control, tramadol (50 mg/kg daily for 21 days with a standard diet), and Tramadol + BB (same tramadol dose plus a diet containing 4% BB for 21 days). Behavioral tests—including the Y-maze, tail suspension, elevated plus maze, and Morris water maze—were conducted. Electrophysiological recordings, immunohistochemistry for GFAP, stereological analysis, neuronal spatial arrangement, and Western blotting were also performed. The BB-supplemented diet significantly improved memory performance in the Tramadol + BB group compared to tramadol-only rats. Field potential analysis showed recovery of the fEPSP slope to near-control levels. Western blot results indicated increased expression of brain-derived neurotrophic factor (BDNF) and repressor element 1-silencing transcription factor (REST) in the hippocampus. BB also reduced tramadol-induced astrogliosis, although astrocyte morphology was largely unchanged except for a decrease in branch number. Additionally, BB enhanced glutathione (GSH) levels and reduced reactive oxygen species (ROS). These findings suggest that BB mitigates tramadol-induced neurotoxicity, potentially through its antioxidant properties and modulation of REST and BDNF pathways. Further research is needed to clarify the molecular mechanisms underlying these protective effects. © King Abdulaziz City for Science and Technology 2026.

Related Docs

View other Related Docs

1. Coenzyme Q10 Attenuates Neurodegeneration in the Cerebellum Induced by Chronic Exposure to Tramadol, Journal of Chemical Neuroanatomy (2024)

2. Effect of Tramadol on Apoptosis and Synaptogenesis in Hippocampal Neurons: The Possible Role of Μ-Opioid Receptor, Drug Development Research (2022)

3. Tramadol Induces Changes in Δ-Fosb, Μ-Opioid Receptor, and P-Creb Level in the Nucleus Accumbens and Prefrontal Cortex of Male Wistar Rat, American Journal of Drug and Alcohol Abuse (2019)

Experts (# of related papers)

View all Related Experts

Saereh Hosseindoost (1)

Mahmoud Reza Hadjighassem (1)

Style	Citing Format
MLA	Nasab AS, et al.. "The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies." 3 Biotech, vol. 16, no. 5, 2026, pp. -.
APA	Nasab AS, Dalband AM, Mohammadzadeh I, Lajevardi Z, Moafi M, Ebrahimi MJ, Nourirad SN, Asadi A, Efazati A, Beirami A, Hasanzadeh M, Nikoohemmat M, Zeidabadi AA, Alinezhad A, Zolfaghari F, Hajibeygi R, Vakili K, Bayat AH, Parvardeh S, ... Aliaghaei A (2026). The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies. 3 Biotech, 16(5), -.
Chicago	Nasab AS, Dalband AM, Mohammadzadeh I, Lajevardi Z, Moafi M, Ebrahimi MJ, Nourirad SN, et al.. "The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies." 3 Biotech 16, no. 5 (2026): -.
Harvard	Nasab AS et al. (2026) 'The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies', 3 Biotech, 16(5), pp. -.
Vancouver	Nasab AS, Dalband AM, Mohammadzadeh I, Lajevardi Z, Moafi M, Ebrahimi MJ, et al.. The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies. 3 Biotech. 2026;16(5):-.
BibTex	@article{ author = {Nasab AS and Dalband AM and Mohammadzadeh I and Lajevardi Z and Moafi M and Ebrahimi MJ and Nourirad SN and Asadi A and Efazati A and Beirami A and Hasanzadeh M and Nikoohemmat M and Zeidabadi AA and Alinezhad A and Zolfaghari F and Hajibeygi R and Vakili K and Bayat AH and Parvardeh S and Farahani RM and Sarkala HB and Sani M and Moghaddam MH and Aliaghaei A}, title = {The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies}, journal = {3 Biotech}, volume = {16}, number = {5}, pages = {-}, year = {2026} }
RIS	TY - JOUR AU - Nasab AS AU - Dalband AM AU - Mohammadzadeh I AU - Lajevardi Z AU - Moafi M AU - Ebrahimi MJ AU - Nourirad SN AU - Asadi A AU - Efazati A AU - Beirami A AU - Hasanzadeh M AU - Nikoohemmat M AU - Zeidabadi AA AU - Alinezhad A AU - Zolfaghari F AU - Hajibeygi R AU - Vakili K AU - Bayat AH AU - Parvardeh S AU - Farahani RM AU - Sarkala HB AU - Sani M AU - Moghaddam MH AU - Aliaghaei A TI - The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies JO - 3 Biotech VL - 16 IS - 5 SP - EP - PY - 2026 ER -

Style

Citing Format

MLA

Nasab AS, et al.. "The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies." 3 Biotech, vol. 16, no. 5, 2026, pp. -.

APA

Nasab AS, Dalband AM, Mohammadzadeh I, Lajevardi Z, Moafi M, Ebrahimi MJ, Nourirad SN, Asadi A, Efazati A, Beirami A, Hasanzadeh M, Nikoohemmat M, Zeidabadi AA, Alinezhad A, Zolfaghari F, Hajibeygi R, Vakili K, Bayat AH, Parvardeh S, ... Aliaghaei A (2026). The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies. 3 Biotech, 16(5), -.

Chicago

Nasab AS, Dalband AM, Mohammadzadeh I, Lajevardi Z, Moafi M, Ebrahimi MJ, Nourirad SN, et al.. "The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies." 3 Biotech 16, no. 5 (2026): -.

Harvard

Nasab AS et al. (2026) 'The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies', 3 Biotech, 16(5), pp. -.

Vancouver

Nasab AS, Dalband AM, Mohammadzadeh I, Lajevardi Z, Moafi M, Ebrahimi MJ, et al.. The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies. 3 Biotech. 2026;16(5):-.

BibTex

@article{ author = {Nasab AS and Dalband AM and Mohammadzadeh I and Lajevardi Z and Moafi M and Ebrahimi MJ and Nourirad SN and Asadi A and Efazati A and Beirami A and Hasanzadeh M and Nikoohemmat M and Zeidabadi AA and Alinezhad A and Zolfaghari F and Hajibeygi R and Vakili K and Bayat AH and Parvardeh S and Farahani RM and Sarkala HB and Sani M and Moghaddam MH and Aliaghaei A}, title = {The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies}, journal = {3 Biotech}, volume = {16}, number = {5}, pages = {-}, year = {2026} }

RIS

TY - JOUR
AU - Nasab AS
AU - Dalband AM
AU - Mohammadzadeh I
AU - Lajevardi Z
AU - Moafi M
AU - Ebrahimi MJ
AU - Nourirad SN
AU - Asadi A
AU - Efazati A
AU - Beirami A
AU - Hasanzadeh M
AU - Nikoohemmat M
AU - Zeidabadi AA
AU - Alinezhad A
AU - Zolfaghari F
AU - Hajibeygi R
AU - Vakili K
AU - Bayat AH
AU - Parvardeh S
AU - Farahani RM
AU - Sarkala HB
AU - Sani M
AU - Moghaddam MH
AU - Aliaghaei A
TI - The Black Barberry Diet Decreases Oxidative Stress and Improves Behavioral Functions in Adult Male Rats Induced Tramadol Neurodegeneration: Behavioral, Molecular and Histopathological Studies
JO - 3 Biotech
VL - 16
IS - 5
SP -
EP -
PY - 2026
ER -

Science Communicator Platform

Authors

Abstract