Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Tramadol Induces Changes in Δ-Fosb, Μ-Opioid Receptor, and P-Creb Level in the Nucleus Accumbens and Prefrontal Cortex of Male Wistar Rat Publisher Pubmed



Sadatshirazi MS1, 2 ; Babhadiashar N1 ; Khalifeh S3 ; Mahboubi S2 ; Ahmadianmoghaddam H1 ; Zarrindast MR1, 4, 5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  4. 4. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Endocrinology and Metabolism Research Institute, Tehran University of Medical Science, Tehran, Iran

Source: American Journal of Drug and Alcohol Abuse Published:2019


Abstract

Background: Besides the analgesic effect of tramadol, prolonged exposure to tramadol can induce adaptive changes thereby leading to dependence and tolerance. Tramadol induces its effect via µ-opioid receptor (MOR). However, tramadol has other targets such as serotonin and epinephrine transporters. Objective: CREB and ΔFosB are transcriptional factors, which are involved in the behavioral abnormalities underlying drug abuse. In this study, the effects of acute and chronic tramadol treatments on MOR, ΔFosB, and CREB levels were studied. Methods: For this purpose, 36 male Wistar rats were used. The animals were divided into two main groups. A total of 18 animals received tramadol (0, 5, and 10 mg/kg) acutely and 18 animals received the same doses for the following 14 days. One hour after the last injection, the NAC and PFC were dissected and kept at −80°C in liquid nitrogen. Using western blotting technique, the levels of MOR, ΔFosB, and p-CREB were evaluated. Results: In the NAC, acute tramadol exposure increases the levels of MOR and p-CREB. Moreover, chronic tramadol administration in this region results in elevated levels of MOR, ΔFosB and p-CREB compared with saline-treated rats. The levels of MOR and p-CREB in the PFC increased in both acute and chronic tramadol exposure. Also, ΔFosB levels increased only following chronic tramadol administration. The results revealed that adaptive changes occurred during drug exposure. Conclusion: We concluded that both CREB and ΔFosB played a role in tramadol dependence. Additionally, increased MOR levels during tramadol treatments might be due to receptor desensitization. © 2018, © 2018 Taylor & Francis, Group, LLC.
Related Docs
1. The Role of Cannabinoid 1 Receptor in the Nucleus Accumbens on Tramadol Induced Conditioning and Reinstatement, Life Sciences (2020)
2. Tramadol, Usage, Misuse, and Addiction Processes, Neuropathology of Drug Addictions and Substance Misuse Volume 3: General Processes and Mechanisms# Prescription Medications# Caffeine and Areca# Polydrug Misuse# Emerging Addictions and Non-Drug Addictions (2016)
3. The Role of Lateral Habenula Nmda Receptors in Tramadol-Induced Conditioning, Behavioural Pharmacology (2023)
4. Acute and Chronic Tramadol Treatment Impresses Tyrosine Kinase B (Trk-B) Receptor in the Amygdala and Nucleus Accumbens, Journal of Iranian Medical Council (2018)
5. Tramadol Use and Public Health Consequences in Iran: A Systematic Review and Meta-Analysis, Addiction (2020)
6. Heroin-Based Crack Induces Hyperalgesia Through Β-Arrestin 2 Redistribution and Phosphorylation of Erk1/2 and Jnk in the Periaqueductal Gray Area, Neuroscience Letters (2019)
7. Exercise Can Restore Behavioural and Molecular Changes of Intergenerational Morphine Effects, Addiction Biology (2022)
8. Possible Involvement of Nucleus Accumbens D1-Like Dopamine Receptors in the Morphine-Induced Condition Place Preference in the Offspring of Morphine Abstinent Rats, Life Sciences (2019)
Experts (# of related papers)
View all Related Experts
Jaleh Gholami (1)
Masoumeh Amin-Esmaeili (1)