Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells

Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells Publisher Pubmed

Fahimipour F^{1, 5} ; Dashtimoghadam E¹ ; Rasoulianboroujeni M¹ ; Yazdimamaghani M² ; Khoshroo K¹ ; Tahriri M¹ ; Yadegari A¹ ; Gonzalez JA¹ ; Vashaee D³ ; Lobner DC⁴ ; Jafarzadeh Kashi TS⁵ ; Tayebi L^{1, 6}

Show Affiliations

1. Marquette University School of Dentistry, Milwaukee, 53233, WI, United States
2. School of Chemical Engineering, Oklahoma State University, Stillwater, 74078, OK, United States
3. Electrical and Computer Engineering Department, North Carolina State University, Raleigh, 27606, NC, United States
4. Department of Biomedical Sciences, Marquette University, Milwaukee, 53233, WI, United States
5. Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, United Kingdom

Source: Dental Materials Published:2018

Abstract

Objective: A systematic characterization of hybrid scaffolds, fabricated based on combinatorial additive manufacturing technique and freeze-drying method, is presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs). Methods: The scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The developed construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the Collagen extracellular matrix. The β-TCP precursor formulations were investigated for their flow-ability at various temperatures, which optimized for fabrication of 3D printed scaffolds with interconnected porosity. The hybrid constructs were characterized by 3D laser scanning microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and compressive strength testing. Results: The in vitro characterization of scaffolds revealed that the hybrid β-TCP/Collagen constructs offer superior DPCs proliferation and alkaline phosphatase (ALP) activity compared to the 3D-printed β-TCP scaffold over three weeks. Moreover, it was found that the incorporation of TCP into the Collagen matrix improves the ALP activity. Significance: The presented results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration. © 2017

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Tahereh Sadat Jafarzadeh Kashi (3)

Reza Masaeli (2)

Style	Citing Format
MLA	Fahimipour F, et al.. "Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells." Dental Materials, vol. 34, no. 2, 2018, pp. 209-220.
APA	Fahimipour F, Dashtimoghadam E, Rasoulianboroujeni M, Yazdimamaghani M, Khoshroo K, Tahriri M, Yadegari A, Gonzalez JA, Vashaee D, Lobner DC, Jafarzadeh Kashi TS, Tayebi L (2018). Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells. Dental Materials, 34(2), 209-220.
Chicago	Fahimipour F, Dashtimoghadam E, Rasoulianboroujeni M, Yazdimamaghani M, Khoshroo K, Tahriri M, Yadegari A, et al.. "Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells." Dental Materials 34, no. 2 (2018): 209-220.
Harvard	Fahimipour F et al. (2018) 'Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells', Dental Materials, 34(2), pp. 209-220.
Vancouver	Fahimipour F, Dashtimoghadam E, Rasoulianboroujeni M, Yazdimamaghani M, Khoshroo K, Tahriri M, et al.. Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells. Dental Materials. 2018;34(2):209-220.
BibTex	@article{ author = {Fahimipour F and Dashtimoghadam E and Rasoulianboroujeni M and Yazdimamaghani M and Khoshroo K and Tahriri M and Yadegari A and Gonzalez JA and Vashaee D and Lobner DC and Jafarzadeh Kashi TS and Tayebi L}, title = {Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells}, journal = {Dental Materials}, volume = {34}, number = {2}, pages = {209-220}, year = {2018} }
RIS	TY - JOUR AU - Fahimipour F AU - Dashtimoghadam E AU - Rasoulianboroujeni M AU - Yazdimamaghani M AU - Khoshroo K AU - Tahriri M AU - Yadegari A AU - Gonzalez JA AU - Vashaee D AU - Lobner DC AU - Jafarzadeh Kashi TS AU - Tayebi L TI - Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells JO - Dental Materials VL - 34 IS - 2 SP - 209 EP - 220 PY - 2018 ER -

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