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Comparative Gene-Expression Profiling of Cd133+ and Cd133- D10 Melanoma Cells Publisher Pubmed



Roudi R1, 2 ; Ebrahimi M3 ; Sabet MN2 ; Najafi A4 ; Nourani MR5 ; Fomeshi MR3 ; Samadikuchaksaraei A6, 7 ; Shariftabrizi A8 ; Madjd Z1, 2
Authors
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Authors Affiliations
  1. 1. Oncopathology Research Center, Iran University of Medical Sciences, Hemmat Street (Highway), Next to Milad Tower, Tehran, 14496-14530, Iran
  2. 2. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  4. 4. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  5. 5. Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  6. 6. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Nuclear Medicine and Molecular Imaging, State University of New York, Buffalo, NY, United States

Source: Future Oncology Published:2015


Abstract

Aims: The present study aimed to compare the gene-expression profiling of CD133+ and CD133- D10 cells. Materials & methods: Cancer stem cell-like properties and gene-expression profiling of CD133+ D10 cells versus CD133- cells were evaluated. Results: The CD133+ D10 cells showed significantly higher clonogenic and spheroid forming potential, also higher expression of stemness genes NANOG and OCT4A compared with the CD133- cells. Gene-expression profiling of CD133+ versus CD133- D10 cells revealed that 130 genes including ABC transporter superfamily (ABCC1, ABCG2 and ABCC6) were upregulated, while 61 genes including apoptosis modifying genes (CASP8 and TNFRSF4) were downregulated. Conclusion: We conclude that many genes involved in drug resistance and tumor aggressiveness are upregulated in CD133+ D10 cells and targeting them might be an efficient strategy for treatment of melanoma. © 2015 Future Medicine Ltd.