Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction

Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction Publisher Pubmed

Salehi Namini M^{1, 4} ; Khanmohammadi M² ; Beheshtizadeh N^{3, 4} ; Najafi MS⁵ ; Heiranitabasi A⁶ ; Ayati A⁵ ; Boroumand S⁵ ; Pournemati B⁷ ; Ai J¹ ; Ebrahimibarough S¹ ; Montazerghaem H⁸ ; Ahmadi Tafti SH⁵

Show Affiliations

1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Biomaterials Group, Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Woloska 141, Warsaw, 02-507, Poland
3. Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4. Regenerative Medicine Group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran
5. Research Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran
8. Cardiovascular Research center, Hormozgan University of Medical Science, Bandar Abbas, Iran

Source: International Journal of Biological Macromolecules Published:2025

Abstract

Therapeutic efficacy of human endometrial stem cells (hEnSCs) encapsulated in hyaluronic acid (HA)-based microcapsules for cardiac regeneration in a rat model of MI is investigated. Cell-enclosed microcapsules were made by loading hEnSCs within hydrogel membrane produced from modified HA possessing phenolic hydroxyl moieties (HA-Ph). The hEnSC-loaded HA-Ph microcapsules (≈150 μm) injected intramyocardially into the peri-infarct area post-MI. The encapsulated cells showed mechanical stability and >87 % cell viability with cellular aggregation in size of about 100 μm until 7 days of culture. Transthoracic echocardiography evaluation indicated a significant increase in ejection fraction in encapsulated cells, compared to the other groups. Histological investigation of fibrosis and scar area by Masson trichrome and hematoxylin and eosin (H&E) staining illustrated less fibrosis and scarring area in the encapsulated cell group compared with the other groups. Furthermore, the cell-laden microcapsules significantly enhance expression intensities of actin and troponin as well as vascular endothelial-specific marker, all of which promote cardiac functions and contribute to a better therapeutic effect than the free-cell injection group in a rat model of MI. Our findings demonstrated that both hEnSCs and specifically hEnSC-loaded HA-based hydrogel vehicle can provide a promising novel therapy for functional restoration in MI instances. © 2025

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Style	Citing Format
MLA	Salehi Namini M, et al.. "Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction." International Journal of Biological Macromolecules, vol. 304, no. , 2025, pp. -.
APA	Salehi Namini M, Khanmohammadi M, Beheshtizadeh N, Najafi MS, Heiranitabasi A, Ayati A, Boroumand S, Pournemati B, Ai J, Ebrahimibarough S, Montazerghaem H, Ahmadi Tafti SH (2025). Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction. International Journal of Biological Macromolecules, 304(), -.
Chicago	Salehi Namini M, Khanmohammadi M, Beheshtizadeh N, Najafi MS, Heiranitabasi A, Ayati A, Boroumand S, et al.. "Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction." International Journal of Biological Macromolecules 304, no. (2025): -.
Harvard	Salehi Namini M et al. (2025) 'Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction', International Journal of Biological Macromolecules, 304(), pp. -.
Vancouver	Salehi Namini M, Khanmohammadi M, Beheshtizadeh N, Najafi MS, Heiranitabasi A, Ayati A, et al.. Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction. International Journal of Biological Macromolecules. 2025;304():-.
BibTex	@article{ author = {Salehi Namini M and Khanmohammadi M and Beheshtizadeh N and Najafi MS and Heiranitabasi A and Ayati A and Boroumand S and Pournemati B and Ai J and Ebrahimibarough S and Montazerghaem H and Ahmadi Tafti SH}, title = {Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction}, journal = {International Journal of Biological Macromolecules}, volume = {304}, number = {}, pages = {-}, year = {2025} }
RIS	TY - JOUR AU - Salehi Namini M AU - Khanmohammadi M AU - Beheshtizadeh N AU - Najafi MS AU - Heiranitabasi A AU - Ayati A AU - Boroumand S AU - Pournemati B AU - Ai J AU - Ebrahimibarough S AU - Montazerghaem H AU - Ahmadi Tafti SH TI - Injectable Hyaluronic Acid-Based Microcapsules Loaded With Human Endometrial Stem Cells Improves Cardiac Function After Myocardial Infarction JO - International Journal of Biological Macromolecules VL - 304 IS - SP - EP - PY - 2025 ER -

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