Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking

Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking Publisher Pubmed

Khanmohammadi M¹ ; Nemati S² ; Ai J¹ ; Khademi F³

Show Affiliations

1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medical Sciences, Tehran University of Medical Sciences, Tehran, 1417743361, Iran
2. Chemical Engineering Faculty, Sahand University of Technology, P.O. Box: 51335/1996, Tabriz, Iran
3. Department of Tissue Engineering & Applied Cell Sciences, School of Advanced Technologies in Medical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Materials Science and Engineering C Published:2019

Abstract

Hydrogel fibers are structurally and biologically useful devices for differentiation of stem cells and fabrication of filament-like tissues. We established cell-laden degradable hydrogel fibers through visible light-initiated crosslinking to differentiate stem cells and fabricate filament-like tissue. Human adipose stem cell (hADSC)-laden fibers were fabricated by cross-linking phenolic-substituted alginate and gelatin (Alg-Ph and Gela-Ph respectively) in an aqueous solution containing cells. The crosslinking of phenolic moieties was mediated by ruthenium(II) tris-bipyridyl dication (Ru(II) bpy and sodium ammonium persulfate (SPS) and irradiating visible light. The hydrogel microfiber fabricated with desirable geometries and dimensions. The encapsulated hADSCs proliferated and grew within hydrogel microfiber, maintained their multipotency ability and formed filament-like constructs. The filament-like tissues covered with an additional heterogeneous cell layer was made by degrading the fiber membrane using alginate-lyase after covering the fiber surface with vascular endothelial cells. Cellular viability is preserved during Alg-Ph and Gela-Ph hydrogel fiber fabrication and filament-like tissue formation. These results demonstrate the feasibility of Alg-based hydrogel fibers obtained through the Ru/SPS-mediated crosslinking system and visible light irradiation for the engineering of filament-like tissues and cell-based therapeutic treatments. © 2019

Related Docs

View other Related Docs

1. Encapsulation of Stem Cells, Principles of Biomaterials Encapsulation: Volume 2 (2023)

2. Cell Encapsulation in Core-Shell Microcapsules Through Coaxial Electrospinning System and Horseradish Peroxidase-Catalyzed Crosslinking, Biomedical Physics and Engineering Express (2020)

3. Hyaluronic Acid/Gelatin Microcapsule Functionalized With Carbon Nanotube Through Laccase-Catalyzed Crosslinking for Fabrication of Cardiac Microtissue, Journal of Biomedical Materials Research - Part A (2022)

4. Characterization of Encapsulated Cells Within Hyaluronic Acid and Alginate Microcapsules Produced Via Horseradish Peroxidase-Catalyzed Crosslinking, Journal of Biomaterials Science# Polymer Edition (2019)

5. Layered Dermal Reconstitution Through Epigallocatechin 3-Gallate Loaded Chitosan Nanoparticle Within Enzymatically Crosslinked Polyvinyl Alcohol/Collagen Fibrous Mat, Journal of Biomaterials Applications (2022)

6. Myocardial Cell Signaling During the Transition to Heart Failure: Cellular Signaling and Therapeutic Approaches, Comprehensive Physiology (2019)

7. Impact of Exosome-Loaded Chitosan Hydrogel in Wound Repair and Layered Dermal Reconstitution in Mice Animal Model, Journal of Biomedical Materials Research - Part A (2020)

8. Production of Uniform Size Cell-Enclosing Silk Derivative Vehicles Through Coaxial Microfluidic Device and Horseradish Crosslinking Reaction, European Polymer Journal (2022)

Experts (# of related papers)

View all Related Experts

Somayeh Ebrahimi Barough (2)

Naghmeh Bahrami (1)

Style	Citing Format
MLA	Khanmohammadi M, et al.. "Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking." Materials Science and Engineering C, vol. 103, no. , 2019, pp. -.
APA	Khanmohammadi M, Nemati S, Ai J, Khademi F (2019). Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking. Materials Science and Engineering C, 103(), -.
Chicago	Khanmohammadi M, Nemati S, Ai J, Khademi F. "Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking." Materials Science and Engineering C 103, no. (2019): -.
Harvard	Khanmohammadi M et al. (2019) 'Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking', Materials Science and Engineering C, 103(), pp. -.
Vancouver	Khanmohammadi M, Nemati S, Ai J, Khademi F. Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking. Materials Science and Engineering C. 2019;103():-.
BibTex	@article{ author = {Khanmohammadi M and Nemati S and Ai J and Khademi F}, title = {Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking}, journal = {Materials Science and Engineering C}, volume = {103}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Khanmohammadi M AU - Nemati S AU - Ai J AU - Khademi F TI - Multipotency Expression of Human Adipose Stem Cells in Filament-Like Alginate and Gelatin Derivative Hydrogel Fabricated Through Visible Light-Initiated Crosslinking JO - Materials Science and Engineering C VL - 103 IS - SP - EP - PY - 2019 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract