Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3

Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3 Publisher Pubmed

Kourosharami M^{1, 2} ; Javan M² ; Semnanian S²

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1. Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
2. Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Source: Journal of Chemical Neuroanatomy Published:2020

Abstract

Noradrenergic neurons of the locus coeruleus (LC) receive projection from hypothalamus orexinergic neurons and express orexin 1 receptor (Orx1). Orx in the locus coeruleus nucleus is involved in the development of morphine dependence. The downstream signaling of Orx contribution to the development of morphine dependence in LC neurons of morphine-dependent rats was studied. Therefore, we evaluated cyclic adenosine monophosphate (cAMP), cAMP response element-binding protein (CREB) and phospholipase Cβ3 (PLCβ3) levels by the application of immunohistochemistry. Results showed that cAMP, CREB and PLCβ3 levels were suppressed by the application of SB-334867 (as a selective Orx1 antagonist) in morphine-dependent rats. Our results unraveled that Orx1 blockade is involved in the development of morphine dependency through diminution of a variety of intracellular events including the cAMP, CREB and PLCβ3 levels in morphine-dependent rats. Furthermore, the Orx1 blockade could decrease the percentage of tyrosine hydroxylase (TH)+/CREB+ and TH+/PLCβ3+ neurons in LC of morphine-treated rats. It is concluded that the activation of Orx1 in LC nucleus might be involved in some intracellular changes in morphine dependent rats. © 2020

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Style	Citing Format
MLA	Kourosharami M, Javan M, Semnanian S. "Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3." Journal of Chemical Neuroanatomy, vol. 108, no. , 2020, pp. -.
APA	Kourosharami M, Javan M, Semnanian S (2020). Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3. Journal of Chemical Neuroanatomy, 108(), -.
Chicago	Kourosharami M, Javan M, Semnanian S. "Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3." Journal of Chemical Neuroanatomy 108, no. (2020): -.
Harvard	Kourosharami M, Javan M, Semnanian S (2020) 'Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3', Journal of Chemical Neuroanatomy, 108(), pp. -.
Vancouver	Kourosharami M, Javan M, Semnanian S. Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3. Journal of Chemical Neuroanatomy. 2020;108():-.
BibTex	@article{ author = {Kourosharami M and Javan M and Semnanian S}, title = {Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3}, journal = {Journal of Chemical Neuroanatomy}, volume = {108}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Kourosharami M AU - Javan M AU - Semnanian S TI - Inhibition of Orexin Receptor 1 Contributes to the Development of Morphine Dependence Via Attenuation of Camp Response Element-Binding Protein and Phospholipase Cβ3 JO - Journal of Chemical Neuroanatomy VL - 108 IS - SP - EP - PY - 2020 ER -

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