Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients

Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients Publisher

Basimi P¹ ; Farrokhi M² ; Ghanbari M³ ; Manshadi SAD^{2, 4} ; Naghib M⁵ ; Baesi K¹

Show Affiliations

1. Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran
2. Iranian Research Center for HIV/AIDS, Iranian Institute for Reduction of High Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Microbial Biotechnology, Faculty of Biological Science, Tehran North Branch, Islamic Azad University, Tehran, Iran
4. Department of Infectious Diseases and Tropical Medicine, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran

Source: HIV and AIDS Review Published:2023

Abstract

Introduction: Resistance to antiretroviral drugs is a serious problem often related to selective drug-induced pressure and sub-optimal drug dosing. This study aimed to investigate drug resistance-associated mutations in human immunodeficiency virus type 1 (HIV-1) integrase gene caused by the drug pressure of reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs). Material and methods: For this purpose, RNA of 50 HIV-infected patients (25 drug-naive patients and 25 patients under antiretroviral therapy [INI naive]) was extracted and one step RT-nested PCR was carried out on HIV integrase (IN). Then, gene sequences were analyzed to determine sub-types and antiretroviral resistance-associated mutations (RAMs). Results: Phylogenetic analysis revealed that recombinant sub-type CRF35-AD was the most prevalent in all patients (87.2%), followed by A1 sub-type (12.8%). Among the 25 ART-experienced patients, two mutations (N155I, G163R) associated with resistance to integrase inhibitors (INI) were found. Among the 25 naive patients, several polymorphisms were observed, which was also lower in this group than in the ART group. Conclusions: The results of this study indicated that the integrase mutations can be caused by the effect of selective pressure induced by antiviral agents, such as RTIs and PIs. Therefore, examination of the integrase drug resistance mutations is recommended before starting treatment in Iran. © 2023 Termedia Publishing House Ltd.. All rights reserved.

2. Antiretroviral Drug Resistance Mutations in Naive and Experienced Patients in Shiraz, Iran, 2014, Archives of Virology (2016)

3. Plasma and Semen Viral Loads Discordance in Hiv-Positive Patients Receiving Antiretroviral Treatment (Art) in Tehran: An Implication for a Healthy Pregnancy, HIV and AIDS Review (2022)

4. Phylogeny and Drug Resistance of Hiv Pr Gene Among Hiv Patients Receiving Rt Inhibitors in Iran, Asian Pacific Journal of Tropical Biomedicine (2016)

5. Evaluation of Acquired Hiv Drug Resistance Among People Living With Hiv Who Have Taken Antiretroviral Therapy for 9-15 Months in 14 Triangular Clinics in Iran, 2015-2016, Intervirology (2019)

Experts (# of related papers)

Seyed Ahmad Seyed Alinaghi (2)

Style	Citing Format
MLA	Basimi P, et al.. "Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients." HIV and AIDS Review, vol. 22, no. 2, 2023, pp. 99-103.
APA	Basimi P, Farrokhi M, Ghanbari M, Manshadi SAD, Naghib M, Baesi K (2023). Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients. HIV and AIDS Review, 22(2), 99-103.
Chicago	Basimi P, Farrokhi M, Ghanbari M, Manshadi SAD, Naghib M, Baesi K. "Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients." HIV and AIDS Review 22, no. 2 (2023): 99-103.
Harvard	Basimi P et al. (2023) 'Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients', HIV and AIDS Review, 22(2), pp. 99-103.
Vancouver	Basimi P, Farrokhi M, Ghanbari M, Manshadi SAD, Naghib M, Baesi K. Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients. HIV and AIDS Review. 2023;22(2):99-103.
BibTex	@article{ author = {Basimi P and Farrokhi M and Ghanbari M and Manshadi SAD and Naghib M and Baesi K}, title = {Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients}, journal = {HIV and AIDS Review}, volume = {22}, number = {2}, pages = {99-103}, year = {2023} }
RIS	TY - JOUR AU - Basimi P AU - Farrokhi M AU - Ghanbari M AU - Manshadi SAD AU - Naghib M AU - Baesi K TI - Drug-Selective Pressure Effect on Hiv Integrase Mutations in Antiretroviral Naive and Experienced Patients JO - HIV and AIDS Review VL - 22 IS - 2 SP - 99 EP - 103 PY - 2023 ER -

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