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Phylogeny and Drug Resistance of Hiv Pr Gene Among Hiv Patients Receiving Rt Inhibitors in Iran Publisher



Baesi K1 ; Moradbeigi M2 ; Ravanshad M3 ; Baghban A3
Authors
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Authors Affiliations
  1. 1. Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran
  2. 2. Iranian Research Center for HIV/AIDS, Iranian Institute for Reduction of High Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Source: Asian Pacific Journal of Tropical Biomedicine Published:2016


Abstract

Objective: To survey the level and patterns of reverse transcriptase-based drug resistance and subtype distribution among antiretroviral-treated HIV-infected patients receiving only reverse transcriptase inhibitors in Iran. Methods: A total of 25 samples of antiretroviral therapy experienced patients with no history of using protease inhibitors were collected. After RNA extraction, reverse transcriptase-nested PCR was performed. The final products were sequenced and then analysed for drug-resistant mutations and subtypes. Results: No drug resistant mutations were observed among the 25 subjects. The results showed the following subtypes among patients: CRF 35_AD (88%), CRF 28_BF (8%), and CRF 29_BF (4%). Conclusions: A significant increase in drug resistance has been noted in recently-infected patients worldwide. Subtype distributions are needed to perform properly-designed surveillance studies to continuously monitor rates and patterns of transmitted drug resistance and subtypes to help guide therapeutic approaches and limit transmission of these variants. © 2016 Hainan Medical University.