Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor

Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor Publisher Pubmed

Ramshini H¹ ; Mohammadzadeh M² ; Ebrahimhabibi A^{3, 4}

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1. Biology Department, Payam Noor University, Tehran, 19395-4697, Iran
2. Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
3. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
4. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2015

Abstract

Clinical application of curcumin for Alzheimer's disease treatment is severely limited with regard to its poor bioavailability, high rate of metabolism, and instability under neutral condition. In the current study, we designed three compounds in which the diketone moiety of curcumin was replaced by cyclohexanone. In these compounds, the linker length of the molecules was optimal; and substitution of dioxolane for hydroxyl groups on compound 3 should prevent metabolic inactivation. The inhibitory effect of the compounds was investigated against hen egg white lysozyme (HEWL) fibrillation using AFM (atomic force microscope), ThT (thioflavin T) and MTT assay. We found that all three compounds were able to inhibit HEWL aggregation in a dose-dependent manner and inhibit the cytotoxic activity of aggregated HEWL. Docking results demonstrated that the compounds could bind into lysozyme and occupy the whole active site groove. In conclusion, we present chemical analogs of curcumin with various modifications in the spacer and the phenolic rings as improved inhibitors of amyloid aggregation. © 2015 Elsevier B.V.

Style	Citing Format
MLA	Ramshini H, Mohammadzadeh M, Ebrahimhabibi A. "Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor." International Journal of Biological Macromolecules, vol. 78, no. , 2015, pp. 396-404.
APA	Ramshini H, Mohammadzadeh M, Ebrahimhabibi A (2015). Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor. International Journal of Biological Macromolecules, 78(), 396-404.
Chicago	Ramshini H, Mohammadzadeh M, Ebrahimhabibi A. "Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor." International Journal of Biological Macromolecules 78, no. (2015): 396-404.
Harvard	Ramshini H, Mohammadzadeh M, Ebrahimhabibi A (2015) 'Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor', International Journal of Biological Macromolecules, 78(), pp. 396-404.
Vancouver	Ramshini H, Mohammadzadeh M, Ebrahimhabibi A. Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor. International Journal of Biological Macromolecules. 2015;78():396-404.
BibTex	@article{ author = {Ramshini H and Mohammadzadeh M and Ebrahimhabibi A}, title = {Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor}, journal = {International Journal of Biological Macromolecules}, volume = {78}, number = {}, pages = {396-404}, year = {2015} }
RIS	TY - JOUR AU - Ramshini H AU - Mohammadzadeh M AU - Ebrahimhabibi A TI - Inhibition of Amyloid Fibril Formation and Cytotoxicity by a Chemical Analog of Curcumin As a Stable Inhibitor JO - International Journal of Biological Macromolecules VL - 78 IS - SP - 396 EP - 404 PY - 2015 ER -

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