Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies

Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies Publisher Pubmed

Rasoulian B¹ ; Almasi A² ; Hoveizi E³ ; Bagher Z⁴ ; Hayat P¹ ; Joghataei MT¹ ; Rezayat SM⁵ ; Tavakol S¹

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1. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
2. Department of Medical Nanotechnology, Pharmaceutical Sciences Branch, Islamic Azad University Pharmaceutical Sciences Branch, Tehran, Iran
3. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4. ENT and Head & Neck Research Center and Department, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
5. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Cellular Physiology Published:2019

Abstract

Two of the most problematic orthopedic and neurosurgeon visits are associated with spine and craniofacial fractures. Therefore, more attention needs to be paid to finding a medicine to repair these fractures. Amongst the most mysterious herbs, Aloe vera stands out. In the present study, the ameliorating function of A. vera on osteogenesis was studied in vitro and in vivo. Osteoblast-like cells were exposed to A. vera, followed by analysis of cell viability, lactate dehydrogenase release, and intracellular reactive oxygen species (ROS) production. The results showed an enhanced cell biocompatibility in a dose-dependent manner due to attenuated intracellular ROS production. Furthermore, a docking study indicated that the strong affinity of A. vera constituents to type I bone morphogenic protein receptor (BMPR1A) without the involvement of the BMPR1A chain B. The induction of osteogenesis prompts extracellular calcium deposition by osteoblasts, which affirms successful in vitro bone regeneration. However, injection of A. vera in rats with critical size calvarial defects induced Runx2, alkaline phosphatase (ALP), OCN, and BMP2 genes overexpression, which led to the formation of victorious bone with enhanced bone density and ALP activity. It is worthy to note that Aloin has the highest affinity to BMPR1A, whereas there are no reports regarding the impact of Aloenin, Aloesin, and γ-sitosterol on osteogenesis. Furthermore, some of them have antitumor potency, and it might be proposed that they are considered as a bone substitute in the osteotomy site of osteosarcoma with the aim of bone recovery and suppression of osteosarcoma. The whole consequences of this investigation manifests the plausibility of using A. vera as an antioxidant and osteoconductive substitute. © 2019 Wiley Periodicals, Inc.

2. The Impact of the Particle Size of Curcumin Nanocarriers and the Ethanol on Beta_1-Integrin Overexpression in Fibroblasts: A Regenerative Pharmaceutical Approach in Skin Repair and Anti-Aging Formulations, DARU# Journal of Pharmaceutical Sciences (2019)

Experts (# of related papers)

Seyed Mahdi Rezayat Sorkhabadi (2)

Style	Citing Format
MLA	Rasoulian B, et al.. "Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies." Journal of Cellular Physiology, vol. 234, no. 8, 2019, pp. 14246-14258.
APA	Rasoulian B, Almasi A, Hoveizi E, Bagher Z, Hayat P, Joghataei MT, Rezayat SM, Tavakol S (2019). Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies. Journal of Cellular Physiology, 234(8), 14246-14258.
Chicago	Rasoulian B, Almasi A, Hoveizi E, Bagher Z, Hayat P, Joghataei MT, Rezayat SM, Tavakol S. "Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies." Journal of Cellular Physiology 234, no. 8 (2019): 14246-14258.
Harvard	Rasoulian B et al. (2019) 'Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies', Journal of Cellular Physiology, 234(8), pp. 14246-14258.
Vancouver	Rasoulian B, Almasi A, Hoveizi E, Bagher Z, Hayat P, Joghataei MT, et al.. Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies. Journal of Cellular Physiology. 2019;234(8):14246-14258.
BibTex	@article{ author = {Rasoulian B and Almasi A and Hoveizi E and Bagher Z and Hayat P and Joghataei MT and Rezayat SM and Tavakol S}, title = {Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies}, journal = {Journal of Cellular Physiology}, volume = {234}, number = {8}, pages = {14246-14258}, year = {2019} }
RIS	TY - JOUR AU - Rasoulian B AU - Almasi A AU - Hoveizi E AU - Bagher Z AU - Hayat P AU - Joghataei MT AU - Rezayat SM AU - Tavakol S TI - Strong Binding Active Constituents of Phytochemical to Bmpr1a Promote Bone Regeneration: In Vitro, in Silico Docking, and in Vivo Studies JO - Journal of Cellular Physiology VL - 234 IS - 8 SP - 14246 EP - 14258 PY - 2019 ER -

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