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Investigation of the Biological Activity, Mechanical Properties and Wound Healing Application of a Novel Scaffold Based on Lignin-Agarose Hydrogel and Silk Fibroin Embedded Zinc Chromite Nanoparticles Publisher



Eivazzadehkeihan R1 ; Moghim Aliabadi HA2, 3 ; Radinekiyan F1 ; Sobhani M1 ; Maleki A1 ; Madanchi H4, 5 ; Mahdavi M6 ; Shalan AE7, 8
Authors
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Authors Affiliations
  1. 1. Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran
  2. 2. Faculty of Chemistry, K. N. Toosi University of Technology, Tehran, Iran
  3. 3. Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Department of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  5. 5. Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  6. 6. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. BCMaterials, Basque Center for Materials, Applications and Nanostructures, Martina Casiano, UPV/EHU Science Park, Barrio Sarriena s/n, Leioa, 48940, Spain
  8. 8. Central Metallurgical Research and Development Institute (CMRDI), P. O. Box 87, Helwan Cairo, 11421, Egypt

Source: RSC Advances Published:2021


Abstract

Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr2O4) nanoparticles. Considering the cell viability technique, red blood cell hemolysis in addition to anti-biofilm assays, it was determined that after three days, the toxicity of the cross-linked lignin-agarose/SF/ZnCr2O4 nanobiocomposite was less than 13%. Moreover, the small hemolytic effect (1.67%) and high level of prevention in forming a P. aeruginosa biofilm with low OD value (0.18) showed signs of considerable hemocompatibility and antibacterial activity. Besides, according to an in vivo assay study, the wounds of mice treated with the cross-linked lignin-agarose/SF/ZnCr2O4 nanobiocomposite scaffold were almost completely healed in five days. Aside from these biological tests, the structural features were evaluated by FT-IR, EDX, FE-SEM, and TG analyses, as well as swelling ratio, rheological, and compressive mechanical study tests. Additionally, it was concluded that adding silk fibroin and ZnCr2O4 nanoparticles could enhance the mechanical tensile properties of cross-linked lignin-agarose hydrogel, and also an elastic network was characterized for this designed nanobiocomposite. © 2021 The Royal Society of Chemistry.
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