Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study

Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study Publisher Pubmed

Foroughi F¹ ; Amirzargar A^{2, 3} ; Ahmadpoor P⁴ ; Noorbakhsh F² ; Nafar M⁴ ; Yekaninejad MS⁵ ; Hosseinzadeh M² ; Shabaz SK² ; Barabadi M² ; Ghorbanpour M⁵ ; Sarrafnejad A¹

Show Affiliations

1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
4. Chronic Kidney Disease Research Center, Department of Nephrology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Transplant Immunology Published:2016

Abstract

Background Leukocyte infiltration into the graft has pivotal effects on kidney transplantation outcome. The present study sought to determine whether the expression of sequential chemokine receptors on CD4+ and CD8+ T cells in human renal allograft can predict clinical episodes. Methods Blood samples from 52 consecutive renal transplant patients were evaluated at the time of transplantation and at three times (2, 90 and 180 days) after transplantation to analyze the expression of CCR1 and CXCR3 on CD4+ and CD8+ T cells by flowcytometry. A total of 30 biopsies, including protocol biopsy (n = 24) and cause biopsy (n = 6), were investigated according to the Banff criteria. Results The mean percentage of CD4+ and CD8+ T cells expressing CCR1 was significantly increased in patients with allograft dysfunction (n = 25) (p = 0.006, p = 0.004). The mean fluorescence intensity of CXCR3 on CD4+ and CD8+ T cells were found to be significantly higher in graft dysfunction than that in well-functioning grafts. (p < 0.001, p = 0.007). Receiver Operating Characteristic (ROC) Curve Analysis showed that the calculated AUC was 0.86 at the third month for CD4+ CCR1+ and CD8+ CCR1+ (p < 0.001). Multiple logistic regression analysis showed that an increase in CD4+ expressing CXCR3 leads to a lower risk of graft dysfunction (OR = 0.37), while an increase in CD8+ expressing CCR1 results in a higher risk of graft dysfunction (OR = 3.66). Conclusion During renal transplantation, CD4+ and CD8+ T cells expressing CCR1 were increased in patients who developed graft dysfunction. These findings may prospectively predict allograft dysfunction, and help elucidate the underlying pathogenic mechanisms. © 2016

Style	Citing Format
MLA	Foroughi F, et al.. "Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study." Transplant Immunology, vol. 38, no. , 2016, pp. 67-74.
APA	Foroughi F, Amirzargar A, Ahmadpoor P, Noorbakhsh F, Nafar M, Yekaninejad MS, Hosseinzadeh M, Shabaz SK, Barabadi M, Ghorbanpour M, Sarrafnejad A (2016). Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study. Transplant Immunology, 38(), 67-74.
Chicago	Foroughi F, Amirzargar A, Ahmadpoor P, Noorbakhsh F, Nafar M, Yekaninejad MS, Hosseinzadeh M, et al.. "Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study." Transplant Immunology 38, no. (2016): 67-74.
Harvard	Foroughi F et al. (2016) 'Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study', Transplant Immunology, 38(), pp. 67-74.
Vancouver	Foroughi F, Amirzargar A, Ahmadpoor P, Noorbakhsh F, Nafar M, Yekaninejad MS, et al.. Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study. Transplant Immunology. 2016;38():67-74.
BibTex	@article{ author = {Foroughi F and Amirzargar A and Ahmadpoor P and Noorbakhsh F and Nafar M and Yekaninejad MS and Hosseinzadeh M and Shabaz SK and Barabadi M and Ghorbanpour M and Sarrafnejad A}, title = {Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study}, journal = {Transplant Immunology}, volume = {38}, number = {}, pages = {67-74}, year = {2016} }
RIS	TY - JOUR AU - Foroughi F AU - Amirzargar A AU - Ahmadpoor P AU - Noorbakhsh F AU - Nafar M AU - Yekaninejad MS AU - Hosseinzadeh M AU - Shabaz SK AU - Barabadi M AU - Ghorbanpour M AU - Sarrafnejad A TI - Increased Levels of Cd4+ and Cd8+ T Cells Expressing Ccr1 in Patients Developing Allograft Dysfunction; a Cohort Study JO - Transplant Immunology VL - 38 IS - SP - 67 EP - 74 PY - 2016 ER -

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