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Sequential Monitoring of Tim-3 Mrna Expression in Blood and Urine Samples of Renal Transplant Recipients Publisher Pubmed



Shahbaz SK1, 2 ; Barabadi M1 ; Ahmadpour P3 ; Pourrezagholi F3 ; Nafar M3 ; Foroughi F4 ; Hosseinzadeh M5 ; Ghorbanpour M6 ; Yekaninejad MS6 ; Amirzargar A1, 7
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Chronic Kidney Disease Research Center and Department of Nephrology, Shahid Labbafinejad Medical Center and Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
  5. 5. Department of Immunology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran
  6. 6. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Transplant Immunology Published:2019


Abstract

Background: T cell immunoglobulin and mucin domain 3 (TIM-3), as a co-inhibitory receptor expressed on Th1, Th17, CD8T, FoxP3 + Treg and innate immune cells, plays an important role in suppression of T cell-mediated immune responses, tolerance induction and T cell exhaustion. In this study, we evaluated sequential alterations of TIM-3 mRNA expression level in blood and urine samples of renal transplant recipients to predict approaching clinical episodes. Methods: A total of 52 adult renal transplant recipients (31 male and 21 female)were enrolled in this study. All the patients received kidney transplant from living unrelated donors. TIM-3 mRNA expression in peripheral blood mononuclear cells (PBMCs)and urinary cells were quantified using Real Time TaqMan polymerase chain reaction (PCR)at 4 different time points (pre-transplantation, 2, 90 and 180 days post-transplantation). Result: TIM-3 mRNA expression level on days 2, 90 and 180 after transplantation was significantly higher in blood and urine samples of patients with graft dysfunction (GD)compared with patients with well-functioning graft (WFG). Our results also showed a high correlation between blood and urinary level of TIM-3 mRNA expression. The data from Receiver Operating Characteristic (ROC)Curve Analysis showed that blood and urinary TIM-3 mRNA expression level at month 3 and 6 could discriminate graft dysfunction (GD)from well-functioning graft (WFG)with high specificity and sensitivity. Conclusion: Our data suggested that serial monitoring of TIM-3 mRNA level in the blood and urine samples of renal transplant recipients could be a useful non-invasive biomarker for prediction and diagnosis of allograft dysfunction. © 2018 Elsevier B.V.
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