Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function

Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function Publisher Pubmed

Salehi S^{1, 2} ; Shahi A¹ ; Afzali S¹ ; Keshtkar AA³ ; Farashi Bonab S¹ ; Soleymanian T⁴ ; Ansaripour B¹ ; Amirzargar AA¹

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1. Department of Immunology, School of Medicine, Tehran University of Medical Science, Tehran, Iran
2. Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Health Sciences Education Development, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4. Nephrology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: International Immunopharmacology Published:2020

Abstract

Background: The balance between inflammatory and anti-inflammatory responses of the immune system has been demonstrated to determine the fate of transplanted allografts. Here we analyzed CD19+CD24hiCD38hi immature transitional regulatory B (TRB) cells, as well as the gene and protein levels of interleukin (IL)-10 and transforming growth factor (TGF)-β in the three separate groups, include of stable transplanted subjects, chronic antibody-mediated rejection (cAMR) patients, and healthy individuals. Method: Peripheral blood mononuclear cells (PBMCs) from stable subjects (n = 36), cAMR patients (n = 36) and healthy controls (n = 18) were isolated. Flowcytometry was performed for CD19, CD24, and CD38 surface markers. ELISA and quantitative real-time PCR were performed for IL-10 and TGF-β cytokines. Result: The percentages of immature TRB cells were significantly decrease in cAMR patients (0.98%) versus stable recipients (2.81%) and healthy subjects (4.03%) (P = 0.001 and P < 0.001, respectively). Total lymphocytes, circulating B cells, memory and mature subsets of B cells did not show any significant difference between the groups. TGF-β mRNA was 3-fold upregulated in the cAMR group compared to stable patients (P < 0.001.), but without significant alteration at the protein level. Also, long-term survival renal transplant recipients had a higher protein but not mRNA levels of IL-10 than short-term survival renal transplant recipients. Conclusion: It seems that immature TRB cell subpopulation might be a crucial regulator of immune system response and plays an important role in determining the transplantation outcome. Furthermore, immunosuppressive IL-10 and TGF-β cytokines might act as a double sword and can exhibit either pathogenic or protective effects against allograft. © 2020

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Style	Citing Format
MLA	Salehi S, et al.. "Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function." International Immunopharmacology, vol. 86, no. , 2020, pp. -.
APA	Salehi S, Shahi A, Afzali S, Keshtkar AA, Farashi Bonab S, Soleymanian T, Ansaripour B, Amirzargar AA (2020). Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function. International Immunopharmacology, 86(), -.
Chicago	Salehi S, Shahi A, Afzali S, Keshtkar AA, Farashi Bonab S, Soleymanian T, Ansaripour B, Amirzargar AA. "Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function." International Immunopharmacology 86, no. (2020): -.
Harvard	Salehi S et al. (2020) 'Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function', International Immunopharmacology, 86(), pp. -.
Vancouver	Salehi S, Shahi A, Afzali S, Keshtkar AA, Farashi Bonab S, Soleymanian T, et al.. Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function. International Immunopharmacology. 2020;86():-.
BibTex	@article{ author = {Salehi S and Shahi A and Afzali S and Keshtkar AA and Farashi Bonab S and Soleymanian T and Ansaripour B and Amirzargar AA}, title = {Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function}, journal = {International Immunopharmacology}, volume = {86}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Salehi S AU - Shahi A AU - Afzali S AU - Keshtkar AA AU - Farashi Bonab S AU - Soleymanian T AU - Ansaripour B AU - Amirzargar AA TI - Transitional Immature Regulatory B Cells and Regulatory Cytokines Can Discriminate Chronic Antibody-Mediated Rejection From Stable Graft Function JO - International Immunopharmacology VL - 86 IS - SP - EP - PY - 2020 ER -

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