Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property

Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property Publisher Pubmed

Bayrami Z^{1, 2} ; Hajiaghaee R¹ ; Khalighisigaroodi F¹ ; Rahimi R³ ; Farzaei MH⁴ ; Hodjat M^{2, 5} ; Baeeri M² ; Rahimifard M² ; Navaeinigjeh M^{2, 6} ; Abdollahi M^{2, 7}

Show Affiliations

1. Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2. Toxicology and Diseases Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran
3. Department of Persian Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran
4. Kermanshah University of Medical Sciences, Kermanshah, Iran
5. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
7. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
8. The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Ethnopharmacology Published:2018

Abstract

Ethnopharmacological relevance: Tragopogon graminifolius (T. graminifolius) from Asteraceae family has been used as a remedy in Persian traditional medicine for the treatment of various disorders such as wound healing. Aim of the study: The purpose of this study is to investigate the compounds of T. graminifolius, which are responsible for its wound healing activity. Materials and methods: The experiment was performed in three phases; each phase consisted of fractionation of extracts followed by scratch assay. The results of the scratch assay were expressed using scratch closure index (SCI), representing the contraction of scratch. Results: In phase I, Ethyl acetate fraction (E) showed the maximum SCI (61.7 ± 3.5) that was selected for more fractionation in the next phase. In phase II, 12 fractions were obtained and labeled as fractions E- A to L, respectively. Based on the SCI of fractions, EF (SCI=68.9 ± 0.6) was the most active fraction in phase II and selected for further fractionation in phase III. In phase III, 8 fractions were resulted by fractionation of EF and labeled as EF- 1–8. Fraction EF5 with the highest SCI (30.8 ± 3.0) was the most effective fraction and Luteolin was the main component. Luteolin significantly improved viability of fibroblast cells and increased cell population that was accompanied by decreased cell apoptosis. Luteolin-induced cell number increase in the S and G2M phases of the cell cycle, further confirms the proliferative effect of this compound. Conclusion: The results showed that the total extract and fractions of T. graminifolius stimulate proliferation and migration of skin fibroblast cells and Luteolin is one of the active compounds responsible for these effects. © 2018 Elsevier B.V.

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Mohammad Abdollahi (6)

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Style	Citing Format
MLA	Bayrami Z, et al.. "Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property." Journal of Ethnopharmacology, vol. 226, no. , 2018, pp. 48-55.
APA	Bayrami Z, Hajiaghaee R, Khalighisigaroodi F, Rahimi R, Farzaei MH, Hodjat M, Baeeri M, Rahimifard M, Navaeinigjeh M, Abdollahi M (2018). Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property. Journal of Ethnopharmacology, 226(), 48-55.
Chicago	Bayrami Z, Hajiaghaee R, Khalighisigaroodi F, Rahimi R, Farzaei MH, Hodjat M, Baeeri M, Rahimifard M, Navaeinigjeh M, Abdollahi M. "Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property." Journal of Ethnopharmacology 226, no. (2018): 48-55.
Harvard	Bayrami Z et al. (2018) 'Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property', Journal of Ethnopharmacology, 226(), pp. 48-55.
Vancouver	Bayrami Z, Hajiaghaee R, Khalighisigaroodi F, Rahimi R, Farzaei MH, Hodjat M, et al.. Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property. Journal of Ethnopharmacology. 2018;226():48-55.
BibTex	@article{ author = {Bayrami Z and Hajiaghaee R and Khalighisigaroodi F and Rahimi R and Farzaei MH and Hodjat M and Baeeri M and Rahimifard M and Navaeinigjeh M and Abdollahi M}, title = {Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property}, journal = {Journal of Ethnopharmacology}, volume = {226}, number = {}, pages = {48-55}, year = {2018} }
RIS	TY - JOUR AU - Bayrami Z AU - Hajiaghaee R AU - Khalighisigaroodi F AU - Rahimi R AU - Farzaei MH AU - Hodjat M AU - Baeeri M AU - Rahimifard M AU - Navaeinigjeh M AU - Abdollahi M TI - Bio-Guided Fractionation and Isolation of Active Component From Tragopogon Graminifolius Based on Its Wound Healing Property JO - Journal of Ethnopharmacology VL - 226 IS - SP - 48 EP - 55 PY - 2018 ER -

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