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Comparison of Testosterone Extraction From Human Plasma Using Mofs (Mil-53(Al) and Zif-8)-Based D-Μ-Spe Coupled to Hplc–Uv Publisher



Akbari S1 ; Takhvar A1 ; Souri E1 ; Ahmadkhaniha R2 ; Morsali A3 ; Khoshayand MR4 ; Amini M1 ; Taheri A5
Authors
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Authors Affiliations
  1. 1. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Human Ecology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran
  4. 4. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medial Sciences, Tehran, Iran
  5. 5. Department of Chemistry, Ilam Branch, Islamic Azad University, Ilam, Iran

Source: Chromatographia Published:2024


Abstract

A novel technique utilizing metal–organic frameworks (MOFs), namely MIL-53(Al) and ZIF-8, has been developed for the extraction of testosterone from human plasma through dispersive micro-solid phase extraction (D-µ-SPE or DMSPE). The synthesized MOFs were subjected to characterization via Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), and scanning electron microscopy (SEM). The parameters affecting the extraction were optimized by response surface methodology (RSM). The optimal extraction conditions were determined to be 15 min for contact time, 1 mg for MIL-53(Al) amount, and 0.23% (w/v) for NaCl concentration. For ZIF-8, a contact time of 22 min, a sorbent amount of 5.4 mg, and a salt concentration of 0.34% (w/v) were obtained. Batch absorption studies were conducted to find appropriate kinetic and isotherm models. The results indicated that while testosterone absorption was favorable in both cases, MIL-53 (Al) had a higher absorption capacity than ZIF-8. Finally, the proposed method was subjected to analytical validation for determination of testosterone in plasma samples. Good analytical performance was achieved, including a dynamic range of 0.05–1 µg/mL using each of the sorbents. The precision (expressed as the relative standard deviation (RSD)) and accuracy (expressed as the percentage error) of the method for testosterone and MIL-53(Al) were found to be 3.40% and 2.79%, respectively, while for testosterone and ZIF-8, they were 4.24% and 4.22%, respectively. The method effectively extracted 97% and 96% of testosterone from spiked plasma samples using MIL-53(Al) and ZIF-8, respectively. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.