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Comparative Study of Dispersive Micro-Solid Phase Extraction of Nandrolone Using Mil-53(Al) and Zif-8 in Human Plasma Publisher



Akbari S1 ; Takhvar A1 ; Souri E1 ; Ahmadkhaniha R2 ; Morsali A3 ; Khoshayand MR4 ; Amini M1 ; Taheri A5
Authors
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Authors Affiliations
  1. 1. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Human Ecology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran
  4. 4. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Chemistry, Ilam Branch, Islamic Azad University, Ilam, Iran

Source: Journal of Analytical Chemistry Published:2024


Abstract

Abstract: The extraction of nandrolone as a steroid hormone using fast methods is of high importance. This was done by dispersive micro-solid phase extraction in the presence of two widely used organometallic frameworks (MIL-53(Al) and ZIF-8). The extraction steps were optimized by an extensive study on the main factors affecting the absorption/desorption efficiency. The optimum conditions were obtained by design of experiments for the extraction and determination of nandrolone in a blank human plasma matrix. Maximum extraction of nandrolone was achieved in 8.5 min by 2.5 mg of MIL-53(Al) in the solution with 0.89% (w/v) salt concentration, while the maximum extraction by ZIF-8 as another sorbent required 10 min, 4.5 mg of sorbent, and 0.60% (w/v) of salt concentration. Batch experiments were carried out to find appropriate kinetic and isotherm models. Finally, the analytical validation of the proposed method was investigated. The dynamic range of the method was from 0.05 to 1 µg/mL. The precision (expressed as the relative standard deviation, RSD) and accuracy (expressed as the percentage error) of extraction by MIL-53(Al) were found 3.90 and 3.39%, respectively, while those by ZIF-8 were calculated as 4.59 and 4.50%, respectively. The proposed method was successfully applied for the determination of nandrolone in spiked samples, achieving high recovery rates of approximately 96% and 94% using MIL-53(Al) and ZIF-8. © Pleiades Publishing, Ltd. 2024. ISSN 1061-9348, Journal of Analytical Chemistry, 2024, Vol. 79, No. 7, pp. 901–913. Pleiades Publishing, Ltd., 2024.