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Bridging Potential of Taurine-Loading Pcl Conduits Transplanted With Henscs on Resected Sciatic Nerves Publisher



Ai A1 ; Saremi J2 ; Ebrahimibarough S3 ; Fereydouni N4, 5 ; Mahmoodi T1 ; Kazemi Rad N1 ; Sarikhani P1 ; Amidi F6
Authors
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Authors Affiliations
  1. 1. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran
  3. 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Tissue engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
  5. 5. Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
  6. 6. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Regenerative Therapy Published:2022


Abstract

Reconstruction of nerve conduits is a promising method for functional improvement in peripheral nerve repair. Besides choosing of a suitable polymer for conduit construction, adding factors such as Taurine improve a more advantageous microenvironment for defect nerve regeneration. Showing several major biological properties of Taurine, for example, regulation of the osmotic pressure, modulation of neurogenesis, and calcium hemostasis, makes it an appropriate option for repairing of defected nerves. To this, we examined repairing effects of Taurine-loading PCL conduits cultured with human endothelial stem cells (hEnSCs) on resected sciatic nerves. PCL/Taurine/Cell conduits transplanted to a 10-mm sciatic nerve gap. Forty-two wistar rats were randomly divided to seven groups: (1) Normal group, (2) Negative control (NC), (3) Positive control (nerve Autograft group), (4) PCL conduits group (PCL), (5) Taurine loaded PCL conduits group (PCL/Taurine), (6) hEnSCs cultured on the PCL conduits (PCL/Cell), (7) hEnSCs cultured on the PCL/Taurine conduits (PCL/Taurine/Cell). Functional recovery of motor and sensory nerves, the action potential of exciting muscle and motor distal latency has seen in PCL/Taurine/Cell conduits. Histological studies showed also remarkable nerve regeneration and obvious bridging has seen in this group. In conclusion, PCL/Taurine/Cell conduits showing suitable mechanical properties and biocompatibility may improve sciatic nerve regeneration. © 2022 The Japanese Society for Regenerative Medicine
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