In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing

In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing Publisher Pubmed

Akhavantavakoli M¹ ; Fard M¹ ; Khanjani S² ; Zare S³ ; Edalatkhah H² ; Mehrabani D⁴ ; Zarnani AH⁵ ; Shirazi R^{1, 6, 7} ; Kazemnejad S²

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1. Department of Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
2. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
3. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran
4. Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran
5. Nanobiotechnology Research Centre, Avicenna Research Institute, ACECR, Tehran, Iran
6. Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
7. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

Source: Biologicals Published:2017

Abstract

The skin wounds caused by insults should be treated immediately to restore the functions and integrity. Recent studies suggest that stem cells-based therapies may be applicable in wound healing. Newly defined menstrual blood-derived stem cells (MenSCs) show high rate of cell proliferation and trans-differentiation potency to various cell types. However, MenSCs potential to generate keratinocyte for future therapeutic use of skin lesions has been remained to investigate. We cultivated MenSCs in the presence of isolated foreskin derived-keratinocytes using an indirect co-culture system and evaluated efficiency of this protocol to generate keratinocytes using immunofluorescent staining and Real Time PCR technique. Our results showed that differentiated keratinocytes express epidermal/keratinocytes lineage specific markers such as K14, p63, and involucrin at both mRNA and protein levels. Immunofluorescent staining showed the expression of involucrin and K14 in differentiated cells in contrast to undifferentiated cells. Moreover, mRNA expression levels of K14 (11.1 folds, p = 0.001), p63 (10.23 folds, p = 0.001), and involucrin (2.94 folds, p = 0.001) were higher in differentiated MenSCs compared to non-cocultured cells. Therefore, we firstly presented evidence about differentiation capability of MenSCs into epidermal/keratinocytes lineage. Considering the advantages of MenSCs such as great accessibility, these stem cells are promising for stem cells-based therapies of skin defects. © 2017 International Alliance for Biological Standardization

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Amirhasan Zarnani (12)

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Style	Citing Format
MLA	Akhavantavakoli M, et al.. "In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing." Biologicals, vol. 48, no. , 2017, pp. 66-73.
APA	Akhavantavakoli M, Fard M, Khanjani S, Zare S, Edalatkhah H, Mehrabani D, Zarnani AH, Shirazi R, Kazemnejad S (2017). In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing. Biologicals, 48(), 66-73.
Chicago	Akhavantavakoli M, Fard M, Khanjani S, Zare S, Edalatkhah H, Mehrabani D, Zarnani AH, Shirazi R, Kazemnejad S. "In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing." Biologicals 48, no. (2017): 66-73.
Harvard	Akhavantavakoli M et al. (2017) 'In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing', Biologicals, 48(), pp. 66-73.
Vancouver	Akhavantavakoli M, Fard M, Khanjani S, Zare S, Edalatkhah H, Mehrabani D, et al.. In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing. Biologicals. 2017;48():66-73.
BibTex	@article{ author = {Akhavantavakoli M and Fard M and Khanjani S and Zare S and Edalatkhah H and Mehrabani D and Zarnani AH and Shirazi R and Kazemnejad S}, title = {In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing}, journal = {Biologicals}, volume = {48}, number = {}, pages = {66-73}, year = {2017} }
RIS	TY - JOUR AU - Akhavantavakoli M AU - Fard M AU - Khanjani S AU - Zare S AU - Edalatkhah H AU - Mehrabani D AU - Zarnani AH AU - Shirazi R AU - Kazemnejad S TI - In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing JO - Biologicals VL - 48 IS - SP - 66 EP - 73 PY - 2017 ER -

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