A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer

A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer Publisher Pubmed

Hassani S¹ ; Maghsoudi AS¹ ; Akmal MR² ; Rahmani S¹ ; Sarihi P¹ ; Ganjali MR^{2, 3} ; Norouzi P^{2, 3} ; Abdollahi M^{1, 3}

Show Affiliations

1. Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
3. Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Pharmacy and Pharmaceutical Sciences Published:2020

Abstract

Purpose: The current project aimed to design a simple, highly sensitive, and economical label-free electrochemical aptasensor for determination of prostate-specific antigen (PSA), as the gold standard biomarker for prostate cancer diagnosis. The aptasensor was set up using a screen-printed carbon electrode (SPCE) modified by gold nanoparticles (Au NPs) conjugated to thiolated aptamers. Methods: Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were implemented for electrochemical (EC) characterization of the aptasensor. The determination of PSA was also performed through differential pulse voltammetry (DPV) in [Fe (CN)6]3-/4- electrolyte solution. Results: The present aptasensor was shown an outstanding linear response in the concentration range of 1 pg/mL-200 ng/mL with a remarkably lower limit of detection of 0.077 pg/mL. The optimum concentration for PSA separation and the optimum incubation time for antigen-aptamer binding were determined by observing and electing the highest electrochemical responses in a specified time or concentration. Conclusion: According to the results of the specificity tests, the designed aptasensor did not show any significant interactions with other analytes in real samples. Clinical functionality of the aptasensor was appraised in serum samples of healthy individuals and patients examining the PSA level through the fabricated aptasensor and the reference methods. Both methods are comparable in sensitivity. The present fabricated PSA aptasensor with substantial characteristics of ultrasensitivity and cost-effectiveness can be conventionally built and used for the routine check-up of the men for prostate problems. © 2020, Canadian Society for Pharmaceutical Sciences. All rights reserved.

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Shokoufeh Hassani (10)

Mohammad Abdollahi (8)

Style	Citing Format
MLA	Hassani S, et al.. "A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer." Journal of Pharmacy and Pharmaceutical Sciences, vol. 23, no. , 2020, pp. 243-258.
APA	Hassani S, Maghsoudi AS, Akmal MR, Rahmani S, Sarihi P, Ganjali MR, Norouzi P, Abdollahi M (2020). A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer. Journal of Pharmacy and Pharmaceutical Sciences, 23(), 243-258.
Chicago	Hassani S, Maghsoudi AS, Akmal MR, Rahmani S, Sarihi P, Ganjali MR, Norouzi P, Abdollahi M. "A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer." Journal of Pharmacy and Pharmaceutical Sciences 23, no. (2020): 243-258.
Harvard	Hassani S et al. (2020) 'A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer', Journal of Pharmacy and Pharmaceutical Sciences, 23(), pp. 243-258.
Vancouver	Hassani S, Maghsoudi AS, Akmal MR, Rahmani S, Sarihi P, Ganjali MR, et al.. A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer. Journal of Pharmacy and Pharmaceutical Sciences. 2020;23():243-258.
BibTex	@article{ author = {Hassani S and Maghsoudi AS and Akmal MR and Rahmani S and Sarihi P and Ganjali MR and Norouzi P and Abdollahi M}, title = {A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer}, journal = {Journal of Pharmacy and Pharmaceutical Sciences}, volume = {23}, number = {}, pages = {243-258}, year = {2020} }
RIS	TY - JOUR AU - Hassani S AU - Maghsoudi AS AU - Akmal MR AU - Rahmani S AU - Sarihi P AU - Ganjali MR AU - Norouzi P AU - Abdollahi M TI - A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of Psa As a Specific Diagnostic Marker of Prostate Cancer JO - Journal of Pharmacy and Pharmaceutical Sciences VL - 23 IS - SP - 243 EP - 258 PY - 2020 ER -

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