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A Label-Free Electrochemical Aptasensor Based on Screen Printed Carbon Electrodes With Gold Nanoparticles-Polypyrrole Composite for Detection of Cardiac Troponin I Publisher



Eshlaghi SN1 ; Syedmoradi L2 ; Amini A1 ; Omidfar K2
Authors
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Authors Affiliations
  1. 1. Department of Electrical Engineering, College of Technical and Engineering, Islamic Azad University, Tehran, 1468763785, Iran
  2. 2. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, 1411713137, Iran

Source: IEEE Sensors Journal Published:2023


Abstract

Cardiac troponin I (cTnI) is a specific biomarker in the diagnosis of acute myocardial infarction (AMI). In this study, a label-free electrochemical aptasensor was developed for the detection of cTnI using an aptamer probe and conductive polypyrrole (PPy)-gold nanoparticles (AuNPs) as electrode substrate. The surface modification of the electrode was characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV) electrochemical methods, scanning electron microscopy (SEM), atomic force microscopy (AFM), and energy-dispersive X-ray spectroscopy (EDX) analysis. SWV was also used to evaluate the analytical performance of the developed aptasensor using 5 mM [Fe(CN)6]3-/4- as a redox mediator. A detection limit of 25 pg mL-1 was obtained with a linear detection range from 50 to 500 pg mL-1. The proposed aptasensor exhibited good selectivity and appropriate sensitivity for clinical diagnostics compared with enzyme-linked immunosorbent assay (ELISA) analysis, allowing rapid, quantitative, and label-free detection of cardiac markers. © 2001-2012 IEEE.
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1. Recent Advances in the Fabrication of Nano-Aptasensors for the Detection of Troponin As a Main Biomarker of Acute Myocardial Infarction, Critical Reviews in Analytical Chemistry (2023)
2. Electrochemical Immunosensor for Determination of Cardiac Troponin I Using Two-Dimensional Metal-Organic Framework/Fe3o4–Cooh Nanosheet Composites Loaded With Thionine and Pctab/Des Modified Electrode, Talanta (2022)
3. The Recent Progress in the Early Diagnosis of Acute Myocardial Infarction Based on Myoglobin Biomarker: Nano-Aptasensors Approaches, Journal of Pharmaceutical and Biomedical Analysis (2022)
Experts (# of related papers)
Kobra Omidfar (1)