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Novel Label-Free Electrochemical Aptasensor for Determination of Diazinon Using Gold Nanoparticles-Modified Screen-Printed Gold Electrode Publisher Pubmed



Hassani S1, 2 ; Akmal MR3 ; Salekmaghsoudi A2 ; Rahmani S1 ; Ganjali MR3 ; Norouzi P3 ; Abdollahi M1, 2
Authors
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Authors Affiliations
  1. 1. Toxicology and Diseases Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences (TUMS), Tehran, Iran
  2. 2. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran
  3. 3. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran

Source: Biosensors and Bioelectronics Published:2018


Abstract

The present study aimed to develop a highly sensitive label-free electrochemical aptasensor for the detection of Diazinon (DZN), as one of the most widespread organophosphorus compounds. The aptasensor was assembled using screen-printed gold electrode modified by thiolated aptamers which were immobilized on gold nanoparticles (Au NPs). Optimum deposition time, in which the highest electrochemical response occurred, was found in 150 s. Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize electrochemical properties of the novel aptasensor. Electrochemical detection was carried out through differential pulse voltammetry in [Fe(CN)6]3-/4- solution. Fluctuation of the current was examined in the DZN concentration range of 0.1–1000 nM. According to the results, the designed aptasensor provided an extremely lower limit of detection (0.0169 nM) compared with HPLC and other colorimetric techniques for DZN detection. The present highly specific designed aptasensor doesn't interact with other analytes in the real sample. Consequently, the present aptasensor is easy to use and relatively inexpensive with a good sensitivity, stability, and reproducibility for this application. We are now evaluating all approaches to make a portable device for fast and sensitive quantification of DZN and related OPs. © 2018 Elsevier B.V.