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Association Between the Expression Levels of Microrna-101, -103, and -29A With Autotaxin and Lysophosphatidic Acid Receptor 2 Expression in Gastric Cancer Patients Publisher



Tutunchi S1 ; Akhavan S2 ; Panahi G3 ; Zare M4 ; Emami Razavi A5 ; Shirkoohi R6
Authors
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Authors Affiliations
  1. 1. Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Postal Code: 8915173143, Iran
  2. 2. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University (IAU), Tehran, Postal Code: 1477893855, Iran
  3. 3. Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Postal Code: 1417653761, Iran
  4. 4. Recombinant Protein Laboratory, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Postal Code: 7134845794, Iran
  5. 5. Iran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Postal Code: 1419733141, Iran
  6. 6. Cancer Biology Research Center, Cancer Research Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Postal Code: 1419733141, Iran

Source: Journal of Oncology Published:2022


Abstract

Background. Gastric cancer (GC) is regarded as the most prevalent malignancy with the high mortality rate, worldwide. However, gastroscopy, a biopsy of suspected sample, and detecting CEA, CA19-9, and CA72-4 are presently used, but these diagnostic approaches have several limitations. Recently, microRNAs as the most important member of noncoding RNAs (ncRNAs) have received attention; recent evidence demonstrates that they can be used as the promising candidate biomarkers for GC diagnosis. We aimed to investigate the association between the microRNA-29a, -101, and -103 expression and autotaxin (ATX) and lysophosphatidic acid receptor 2 (LPA2) expression in GC patients. Material and Methods. The present study was conducted on 40 paired samples of primary GC tissue and adjacent noncancerous tissue. The gene expression levels of miR-101, -103, -29, ATX, and LPA2 were analyzed by quantitative reverse-transcription PCR (qRT-PCR). Besides, the protein levels of ATX and LPA2 were evaluated using western blot. Results. The expression levels of miR-29 and miR-101 were significantly lower (p value < 0.0001), but the miR-103 and LPA2 were significantly higher in gastric tumor samples compared to the corresponding nontumor tissues (p value < 0.0001). Moreover, the diagnostic accuracy of miRs to discrimine the GC patients from noncancerous controls was reliable (miR-101, sensitivity: 82.5% and specificity: 85%; miR-103, sensitivity: 72.5% and specificity: 90%; miR-29, sensitivity: 77.5% and specificity: 70%). Conclusion. It seems that determining the expression level of miR-101, -103, and -29, as the novel diagnostic biomarkers, has diagnostic value to distinguish GC patients from healthy individuals. © 2022 Sara Tutunchi et al.
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