The Pre-Mir-27A Variant Rs895819 May Contribute to Type 2 Diabetes Mellitus Susceptibility in an Iranian Cohort Publisher Pubmed



Ghaedi H¹ ; Tabasinezhad M² ; Alipoor B³ ; Shokri F¹ ; Movafagh A¹ ; Mirfakhraie R¹ ; Omrani MD¹ ; Masotti A⁴
Source: Journal of Endocrinological Investigation Published:2016

Abstract

Purpose: The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort. Methods: In this case–control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) for genotyping. Results: We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53–0.97). Conclusion: Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes. © 2016, Italian Society of Endocrinology (SIE).