Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Human Menstrual Blood Stem Cell-Derived Granulosa Cells Participate in Ovarian Follicle Formation in a Rat Model of Premature Ovarian Failure in Vivo Publisher Pubmed



Noory P1 ; Navid S2 ; Zanganeh BM1 ; Talebi A3, 4 ; Borhanihaghighi M1 ; Gholami K1 ; Manshadi MD1 ; Abbasi M1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, 0098, Iran
  2. 2. Department of Anatomy, Gonabad University of Medical Sciences, Gonabad, Iran
  3. 3. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  4. 4. Clinical Research Development Unit, Bahar Hospital, Shahroud University of Medical Sciences, Shahroud, Iran

Source: Cellular Reprogramming Published:2019


Abstract

We recently reported the application of human menstrual blood stem cells' (HuMenSCs) transplantation as a treatment modality in a rat model of premature ovarian failure (POF). We continued to investigate further in this respect. Female rats were injected intraperitoneally with 36 mg/kg busulfan. HuMenSCs were obtained, grown, and analyzed for immunophenotypic features at passage three. The cells were labeled with CM-Dil and infused into the rats. There were four groups: normal, negative control, treatment, and Sham. One month after treatment, the ovaries were collected and weighed. Histological sections were prepared from the ovary and HuMenSCs were tracking. Subsequently, we examined the changes of expression of Bax and B cell lymphoma 2 (Bcl2) genes by real-time polymerase chain reaction assay. One month after HuMenSCs transplantation, these cells were located in the ovarian interstitium and granulosa cells (GCs). The number of TUNEL-positive cells significantly decreased in the treatment group. Also the expression level of Bax genes, unlike Bcl2 gene, significantly decreased compared with negative and sham groups. In our study, HuMenSCs were tracked in ovarian tissues within 2 months after transplantation, and they differentiated into GCs. Therefore, the use of these cells can be a practical and low-cost method for the treatment of POF patients. © Copyright 2019, Mary Ann Liebert, Inc., publishers 2019.
Related Docs
View other Related Docs
1. The Effects of Human Menstrual Blood Stem Cells-Derived Granulosa Cells on Ovarian Follicle Formation in a Rat Model of Premature Ovarian Failure, Microscopy Research and Technique (2019)
2. Advances in Stem Cell Therapy Based on Tissue Engineering in the Treatment of Female Infertility With a Focus on Pof, Journal of Kerman University of Medical Sciences (2022)
3. Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application, Frontiers in Cell and Developmental Biology (2020)
4. Tissue Engineering and Stem Cell-Based Therapeutic Strategies for Premature Ovarian Insufficiency, Regenerative Therapy (2024)
5. A Review on Treatment of Premature Ovarian Insufficiency: Characteristics, Limitations, and Challenges of Stem Cell Versus Exosometherapy, Veterinary Medicine International (2023)
6. Protective Role of Stem Cells in Poi: Current Status and Mechanism of Action, a Review Article, Heliyon (2024)
7. Efficient Generation of Functional Hepatocyte-Like Cells From Menstrual Blood-Derived Stem Cells, Journal of Tissue Engineering and Regenerative Medicine (2015)
8. Comparative Restoration of Acute Liver Failure by Menstrual Blood Stem Cells Compared With Bone Marrow Stem Cells in Mice Model, Cytotherapy (2017)
Experts (# of related papers)
View all Related Experts
Amirhasan Zarnani (7)
Mehdi Abbasi (5)
Other Related Docs
9. Mouse Preantral Follicle Growth in 3D Co-Culture System Using Human Menstrual Blood Mesenchymal Stem Cell, Reproductive Biology (2018)
10. Mesenchymal Endometrial Stem/Stromal Cells for Hard Tissue Engineering: A Review of in Vitro and in Vivo Evidence, Regenerative Medicine (2017)
11. Promotion of Excisional Wound Repair by a Menstrual Blood-Derived Stem Cell-Seeded Decellularized Human Amniotic Membrane, Biomedical Engineering Letters (2018)
12. Colonization of Neonate Mouse Spermatogonial Stem Cells Co-Culture With Sertoli Cells in the Presence and Absence Soft Agar, Asian Pacific Journal of Reproduction (2020)
13. Protective Effects of the Antioxidant Capacity of Curcumin Against Apoptosis on the Expression Level of Apoptosis-Related Caspase-3 Gene in Varicocelized Rats, Surgical Chronicles (2021)
14. Characteristic and Regenerative Potential of Human Endometrial Stem Cells and Progenitors, Stem cells: From Potential to Promise (2021)
15. Comparative Capability of Menstrual Blood Versus Bone Marrow Derived Stem Cells in Neural Differentiation, Molecular Biology Reports (2017)
16. Three-Dimensional Co-Culture of Human Spermatogonial Stem Cells With Sertoli Cells in Soft Agar Culture System Supplemented by Growth Factors and Laminin, Acta Histochemica (2020)
17. Purification and Identification of Cd146 Positive Endometrial Somatic Stem Cell From Human Normal Endometrium Biopsy and Gene Expression Criteria, Minerva Biotecnologica (2016)
18. Mesenchymal Stem/Stromal Cells As a Valuable Source for the Treatment of Immune-Mediated Disorders, Stem Cell Research and Therapy (2021)
19. In Vitro Differentiation of Menstrual Blood Stem Cells Into Keratinocytes: A Potential Approach for Management of Wound Healing, Biologicals (2017)
20. Effect of a Freezing Medium Containing Melatonin on Markers of Pre-Meiotic and Post-Meiotic Spermatogonial Stem Cells (Sscs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion, Reproductive Sciences (2021)
21. Sciatic Nerve Regeneration by Transplantation of Menstrual Blood-Derived Stem Cells, Molecular Biology Reports (2017)
22. Extracellular Vesicles Derived From Human Embryonic Stem Cell-Mscs Ameliorate Cirrhosis in Thioacetamide-Induced Chronic Liver Injury, Journal of Cellular Physiology (2018)
23. Application of Stem Cells to Infertility Treatment With Emphasis on Mesenchymal Stem Cells and Ovarian Stem Cells, American Journal of Perinatology (2018)
24. Melatonin in Cryopreservation Media Improves Transplantation Efficiency of Frozen–Thawed Spermatogonial Stem Cells Into Testes of Azoospermic Mice, Stem Cell Research and Therapy (2022)
25. Mutation in Alox12b Likely Cause of Poi and Also Ichthyosis in a Large Iranian Pedigree, Molecular Genetics and Genomics (2020)
26. Astaxanthin Relieves Busulfan-Induced Oxidative Apoptosis in Cultured Human Spermatogonial Stem Cells by Activating the Nrf-2/Ho-1 Pathway, Reproductive Sciences (2022)
27. Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells, Biopreservation and Biobanking (2022)