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High Sensitive Detection of Staphylococcal Enterotoxin B by Silver Enhanced Lateral Flow Assay Publisher



Gholamzad M1 ; Seyedkhan Z2 ; Khamsi HM3 ; Khorshidi M4
Authors
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Authors Affiliations
  1. 1. Department of Microbiology and Immunology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  2. 2. Department of Biology, College of Basic Science, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
  3. 3. Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
  4. 4. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Iranian Journal of Medical Microbiology Published:2020


Abstract

Background: Staphylococcus aureus is one of the most important microorganisms that can colonize the human body and cause various types of human diseases by secreting virulence factors. The lateral flow assay (LFA) is one of the well-known commercial immunoassay methods that provides a high sensitive and rapid approach to monitoring infectious agents in blood, serum and urine. LFA has been considered as an ideal immunochromatographic test. Methods: Anti Staphylococcal Enterotoxin B (SEB) antibodies were conjugated to 20 nm colloidal gold nanoparticles and were applied in assembled lateral flow layer. Suitable reagents were prepared and used in silver enhancement method. We designed a single immunochromatographic test strip to detect SEB. Results: In this study, the smallest amount of SEB identified using sandwich LFA method was 10 ng/mL. We also established a silver enhanced method. Silver could improve the sensitivity detection of the test 100-fold greater than the previously mentioned sandwich LFA. Conclusion: Regarding the high sensitivity of the new method for detection and measurement of SEB (0.1 ng/mL), this strip test offers great promise for a rapid technique instead of the other diagnostic SEB tests in laboratories for the first time. © 2020. This is an original open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License which permits copy and redistribution of the material just in noncommercial usages with proper citation. All rights reserved.
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