Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
An Anticancer Effect of Umbilical Cord-Derived Mesenchymal Stem Cell Secretome on the Breast Cancer Cell Line Publisher Pubmed



Mirabdollahi M1 ; Haghjooyjavanmard S1 ; Sadeghialiabadi H2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Medicinal Chemistry Department, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, 81746-73461, Iran

Source: Cell and Tissue Banking Published:2019


Abstract

Nowadays, Mesenchymal stem cells (MSCs) have become one of the most attractive tools for treating tumors, due to their specific characteristics, the most prominent of which are tropism toward tumor. These cells will exert their effects through their secretion. In this study, our aim was to evaluate the anti-cancer effect of umbilical cord-derived mesenchymal cells (UCMSC) secretome, on MCF-7 tumor cells. MSCs were extracted from the umbilical cord of mothers, having normal delivery or cesarean section. After culture, the supernatants of these cells were collected and freeze-dried. The cytotoxic effect of freeze-dried secretome was examined at different concentrations on MCF-7 and the optimum concentrations (IC50) were calculated, using MTT assay. These results were confirmed by BrdU assay. The effect of induction of apoptosis of the MSC secretome on MCF-7 was determined, using annexin V/PI method by flow cytometry. The results of our study indicate that the isolation and growth time of UCMSCs of mothers who were naturally delivered was lower than those who received cesarean section. Co-culture studies showed that MSCs had cytotoxic effects on MCF-7 cells. The MSC secretome also showed cytotoxic effects on the MCF-7 cell line, this effect was mediated by induction of apoptosis, which was dose-dependent with an IC50 of 10 mg/mL. © 2019, Springer Nature B.V.
Related Docs
View other Related Docs
1. Effect of Human Wharton's Jelly Mesenchymal Stem Cell Secretome on Proliferation, Apoptosis and Drug Resistance of Lung Cancer Cells, Research in Pharmaceutical Sciences (2015)
2. Human Wharton's Jelly Mesenchymal Stem Cells-Derived Secretome Could Inhibit Breast Cancer Growth in Vitro and in Vivo, Iranian Journal of Basic Medical Sciences (2020)
3. Human Wharton's Jelly Mesenchymal Stem Cell Secretome Display Antiproliferative Effect on Leukemia Cell Line and Produce Additive Cytotoxic Effect in Combination With Doxorubicin, Tissue and Cell (2015)
4. Comparison of Human Mesenchymal Stem Cells Isolated by Explant Culture Method From Entire Umbilical Cord and Wharton’S Jelly Matrix, Cell and Tissue Banking (2014)
5. Recent Advancements to Engineer Mesenchymal Stem Cells and Their Extracellular Vesicles for Targeting and Destroying Tumors, Progress in Biophysics and Molecular Biology (2023)
6. Genetically Engineered Mesenchymal Stem Cells: Targeted Delivery of Immunomodulatory Agents for Tumor Eradication, Cancer Gene Therapy (2020)
7. In Vitro Assessment of Cytokine Expression Profile of Mcf-7 Cells in Response to Hwj-Mscs Secretome, Advanced Pharmaceutical Bulletin (2019)
8. Mesenchymal Stem Cells: A New Platform for Targeting Suicide Genes in Cancer, Journal of Cellular Physiology (2018)
Experts (# of related papers)
View all Related Experts
Shaghayegh Haghjooy Javanmard (10)
Rasoul Salehi (6)
Other Related Docs
9. Dual Protective and Cytotoxic Benefits of Mesenchymal Stem Cell Therapy in Combination With Chemotherapy/Radiotherapy for Cancer Patients, Critical Reviews in Eukaryotic Gene Expression (2015)
10. Antiproliferative and Apoptotic Effects of Conditioned Medium Released From Human Amniotic Epithelial Stem Cells on Breast and Cervical Cancer Cells, International Journal of Immunopathology and Pharmacology (2023)
11. Phic31/Piggybac Modified Stromal Stem Cells: Effect of Interferon Γ And/Or Tumor Necrosis Factor (Tnf)-Related Apoptosis-Inducing Ligand (Trail) on Murine Melanoma, Molecular Cancer (2014)
12. Application of Mesenchymal Stem Cells in Melanoma: A Potential Therapeutic Strategy for Delivery of Targeted Agents, Current Medicinal Chemistry (2016)
13. Human Mesenchymal Stromal Cell Therapy for Prevention and Recovery of Chemo/Radiotherapy Adverse Reactions, Cytotherapy (2015)
14. The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model, Cancer Letters (2018)
15. Bypassing the Need for Pre-Sensitization of Cancer Cells for Anticancer Trail Therapy With Secretion of Novel Cell Penetrable Form of Smac From Ha-Mscs As Cellular Delivery Vehicle, Tumor Biology (2015)
16. Method and Key Points for Isolation of Human Amniotic Epithelial Cells With High Yield, Viability and Purity, BMC Research Notes (2017)
17. Pomegranate Seed Extract Enhances the Inhibitory Effect of Adipose- Derived Mesenchymal Stem Cells on Breast Cancer Cell Line in Co-Culture Conditions, Research in Pharmaceutical Sciences (2022)
18. Genetically Modified Murine Adipose-Derived Mesenchymal Stem Cells Producing Interleukin-2 Favor B16f10 Melanoma Cell Proliferation, Immunological Investigations (2015)
19. Non-Viral Delivery Systems for Breast Cancer Gene Therapy, Current Gene Therapy (2017)
20. Exploring the Potential of Montmorillonite As an Antiproliferative Nanoagent Against Mda-Mb-231 and Mcf-7 Human Breast Cancer Cells, Cells (2024)
21. Non-Viral Gene Transfer Into Murine Adipose Derived Mesenchymal Stem Cells: A Comparative Study of Different Non-Viral Methods, Gene Therapy and Molecular Biology (2013)