Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Changes in Protease-Activated Receptor and Trypsin-1 Expression Are Involved in the Therapeutic Effect of Mg2+Supplementation in Type 2 Diabetes-Induced Gastric Injury in Male Adult Rats Publisher



Mehranfard N1 ; Rezazadeh H2 ; Soltani N2 ; Dastgerdi AH3 ; Ghanbari Rad M2 ; Ghasemi M2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
  2. 2. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Preclinical Medicine, School of Medicine, Najafabad Branch, Islamic Azad University, Isfahan, Iran

Source: Advances in Pharmacological and Pharmaceutical Sciences Published:2023


Abstract

Purpose. Gastric inflammation is common and usually severe in patients with type 2 diabetes mellitus (T2DM). Evidence suggests protease-activated receptors (PARs) are a link between inflammation and gastrointestinal dysfunction. Given that magnesium (Mg2+) deficiency is a highly prevalent condition in T2DM patients, we assessed the therapeutic role of Mg2+ on the factors involved in gastric inflammation in T2DM. Methods. A rat model of T2DM gastropathy was established using a long-term high-fat diet + a low dose of streptozocin. Twenty-four rats were divided into control, T2DM, T2DM + insulin (positive control), and T2DM + Mg2+ groups. At the end of 2-month therapies, changes in the expression of gastric trypsin-1, PAR1, PAR2, PAR3, PI3K/Akt, and COX-2 proteins were measured by western blot. Hematoxylin and eosin and Masson's trichrome staining were used to detect gastric mucosal injury and fibrosis. Results. The expression of trypsin-1, PAR1, PAR2, PAR3, and COX-2 increased in diabetes, and Mg2+/insulin treatment strongly decreased their expression. The PI3K/p-Akt significantly decreased in T2DM, and treatment with Mg2+/insulin improved PI3K in T2DM rats. Staining of the gastric antrum tissue of the insulin/Mg2+-treated T2DM rats showed a significantly minimal mucosal and fibrotic injury compared with those of rats from the T2DM group. Conclusion. Mg2+ supplement, comparable to insulin, via decreasing PARs expression, mitigating COX-2 activity, and decreasing collagen deposition could exert a potent gastroprotective effect against inflammation, ulcer, and fibrotic development in T2DM patients. © 2023 Nasrin Mehranfard et al.
Related Docs
View other Related Docs
1. Long-Term Gaba Supplementation Regulates Diabetic Gastroenteropathy Through Gaba Receptor/Trypsin-1/Pars/Akt/Cox-2 Axis, Doklady Biochemistry and Biophysics (2024)
2. The Therapeutic Effects of Magnesium in Insulin Secretion and Insulin Resistance, Advanced Biomedical Research (2022)
3. Combination Therapy With Gaba and Mgso4improves Insulin Sensitivity in Type 2 Diabetic Rat, International Journal of Endocrinology (2022)
4. Magnesium Sulfate Improves Insulin Resistance in High Fat Diet Induced Diabetic Parents and Their Offspring, European Journal of Pharmacology (2021)
5. Unraveling the Mechanisms of Magnesium Supplementation in Alleviating Chronic Kidney Disease Complications and Progression: Balancing Risks and Benefits, Biological Trace Element Research (2024)
6. The Effect of Long-Term Magnesium Treatment on Changes in Hepatic Autophagic and Apoptotic Ability of Type 2 Diabetic Female Rats and Their Offspring, Gene Reports (2024)
7. Impact of Magnesium Sulfate Therapy in Improvement of Renal Functions in High Fat Diet-Induced Diabetic Rats and Their Offspring, Scientific Reports (2023)
8. The Effects of Magnesium Supplementation on Lipid Profile Among Type 2 Diabetes Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials, Biological Trace Element Research (2021)
Experts (# of related papers)
View all Related Experts
Nepton Soltani (13)
Ardeshir Talebi (6)
Other Related Docs
9. Effect of Magnesium Sulfate Administration to Improve Insulin Resistance in Type 2 Diabetes Animal Model: Using the Hyperinsulinemic-Euglycemic Clamp Technique, Fundamental and Clinical Pharmacology (2018)
10. Involvement of Γ-Aminobutyric Acid and N-Methyl-D-Aspartate Receptors in Diabetic Gastropathy in Rats: Possible Beneficial Effect of Prolonged Treatment With Insulin and Magnesium Supplement, Archives of Razi Institute (2024)
11. Effect of Oral Magnesium Sulfate Administration on Blood Glucose Hemostasis Via Inhibition of Gluconeogenesis and Foxo1 Gene Expression in Liver and Muscle in Diabetic Rats, Biomedicine and Pharmacotherapy (2019)
12. Oral Herbal Supplement Containing Magnesium Sulfate Improve Metabolic Control and Insulin Resistance in Non-Diabetic Overweight Patients: A Randomized Double Blind Clinical Trial, Medical Journal of the Islamic Republic of Iran (2019)
13. Effect of Peroxisome Proliferator-Activated Receptor Agonists on Serum Inflammatory Markers in Diabetic Rats, Journal of Isfahan Medical School (2014)
14. The Interaction of Helicobacter Pylori Infection and Type 2 Diabetes Mellitus, Advanced Biomedical Research (2019)
15. Beneficial Effects of Mgso4 on Tfam, Upc3 and Fndc5 Mrna Expressions in Skeletal Muscle of Type 2 Diabetic Rats: A Possible Mechanism to Improve Insulin Resistance, Molecular Biology Reports (2022)
16. Effect of Oral Magnesium Sulfate Administration on Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Gene Expression to Prevent Atherosclerosis in Diabetic Rat Vessels, Journal of Diabetes Investigation (2019)
17. Magnesium Sulfate Administration in Moderate Coronary Artery Disease Patients Improves Atherosclerotic Risk Factors: A Double-Blind Clinical Trial Study, Journal of Cardiovascular Pharmacology (2020)
18. The Effect of Oral Magnesium Supplementation on Inflammatory Biomarkers in Adults: A Comprehensive Systematic Review and Dose–Response Meta-Analysis of Randomized Clinical Trials, Biological Trace Element Research (2022)
19. Effect of Long-Term Administration of Oral Magnesium Sulfate and Insulin to Reduce Streptozotocin-Induced Hyperglycemia in Rats: The Role of Akt2 and Irs1 Gene Expressions, Biological Trace Element Research (2019)