Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Involvement of Γ-Aminobutyric Acid and N-Methyl-D-Aspartate Receptors in Diabetic Gastropathy in Rats: Possible Beneficial Effect of Prolonged Treatment With Insulin and Magnesium Supplement Publisher Pubmed



Saberi H1 ; Mehranfard N2 ; Rezazadeh H3 ; Ghasemi M3
Authors
Show Affiliations
Authors Affiliations
  1. 1. School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Nanokadeh Darooee Samen Private Joint Stock Company, Urmia, 5715793731, Iran
  3. 3. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Archives of Razi Institute Published:2024


Abstract

Gastrointestinal dysfunction is a severe and common complication in diabetic patients. Some evidence shows that gamma-aminobutyric acid (GABA) and glutamate contribute to diabetic gastrointestinal abnormalities. Therefore, we examined the impact of prolonged treatment with insulin and magnesium supplements on the expression pattern of GABA type A (GABA-A), GABA-B, and N-methyl-D-aspartate (NMDA) glutamate receptors as well as nitric oxide synthase 1 (NOS-1) in the stomach of type 2 diabetic rats. Twenty-four male Wistar rats were randomized to four groups (six rats each): 1) control, 2) type 2 diabetes: rats fed with a high-fat diet for three months + a low dose of streptozotocin (35 mg/kg), 3) type 2 diabetes + magnesium, and 4) type 2 diabetes + insulin. The expression of NOS-1, GABA-A, GABA-B, and NMDA receptors was detected using western blotting. The NOS-1 expression was substantially diminished (P<0.01), while the expression of GABA-A (P<0.001), GABA-B (P<0.001), and NMDA (P<0.001) receptors was enhanced in the stomach of diabetic rats relative to control. Treatment with magnesium and insulin improved NOS-1 expression in diabetic rats, although this effect was greater in magnesium treatment alone. Magnesium also restored the expression of GABA-A and GABA-B receptors in diabetic rats to control values. Moreover, insulin treatment improved GABA-A receptor expression in diabetic rats (P<0.05). No considerable alterations were detected in NMDA receptor levels in the treatment groups. The results suggest a significant role of magnesium and insulin in improving gastric motility and secretory disorders associated with diabetes through modifying the expression of GABAergic receptors. Copyright © 2023.
Related Docs
View other Related Docs
1. Long-Term Gaba Supplementation Regulates Diabetic Gastroenteropathy Through Gaba Receptor/Trypsin-1/Pars/Akt/Cox-2 Axis, Doklady Biochemistry and Biophysics (2024)
2. Combination Therapy With Gaba and Mgso4improves Insulin Sensitivity in Type 2 Diabetic Rat, International Journal of Endocrinology (2022)
3. Gaba Dramatically Improves Glucose Tolerance in Streptozotocin-Induced Diabetic Rats Fed With High-Fat Diet, European Journal of Pharmacology (2018)
4. Effect of Long-Term Administration of Oral Magnesium Sulfate and Insulin to Reduce Streptozotocin-Induced Hyperglycemia in Rats: The Role of Akt2 and Irs1 Gene Expressions, Biological Trace Element Research (2019)
5. Gamma-Aminobutyric Acid Attenuates Insulin Resistance in Type 2 Diabetic Patients and Reduces the Risk of Insulin Resistance in Their Offspring, Biomedicine and Pharmacotherapy (2021)
6. Gamma-Aminobutyric Acid Attenuates Insulin Resistance in Type 2 Diabetic Patients and Reduces the Risk of Insulin Resistance in Their Offspring, Koomesh (2023)
7. Effect of Magnesium Sulfate Administration to Improve Insulin Resistance in Type 2 Diabetes Animal Model: Using the Hyperinsulinemic-Euglycemic Clamp Technique, Fundamental and Clinical Pharmacology (2018)
8. An Investigation Into the Effects of Chemical, Pharmaceutical, and Herbal Compounds on Neuroglobin: A Literature Review, Current medicinal chemistry (2024)
Experts (# of related papers)
View all Related Experts
Nepton Soltani (22)
Mohammadreza Sharifi (13)
Other Related Docs
9. The Effect of Long-Term Magnesium Treatment on Changes in Hepatic Autophagic and Apoptotic Ability of Type 2 Diabetic Female Rats and Their Offspring, Gene Reports (2024)
10. Impact of Magnesium Sulfate Therapy in Improvement of Renal Functions in High Fat Diet-Induced Diabetic Rats and Their Offspring, Scientific Reports (2023)
11. Determination of the Extracellular Basal Levels of Glutamate and Gaba at Dentate Gyrus of Streptozotocin-Induced Diabetic Rats, Pathophysiology (2009)
12. Association Between High Doses Consumption of Niacin and Type 2 Diabetes, Journal of Babol University of Medical Sciences (2012)
13. Changes in Protease-Activated Receptor and Trypsin-1 Expression Are Involved in the Therapeutic Effect of Mg2+Supplementation in Type 2 Diabetes-Induced Gastric Injury in Male Adult Rats, Advances in Pharmacological and Pharmaceutical Sciences (2023)
14. Effects of Treadmill Running on Extracellular Basal Levels of Glutamate and Gaba at Dentate Gyrus of Streptozotocin-Induced Diabetic Rats, Journal of Research in Medical Sciences (2010)
15. Effect of Oral Administration of Magnesium on Cisplatin-Induced Nephrotoxicity in Normal and Streptozocin-Induced Diabetic Rats, Nephro-Urology Monthly (2013)
16. Effect of Oral Magnesium Sulfate Administration on Blood Glucose Hemostasis Via Inhibition of Gluconeogenesis and Foxo1 Gene Expression in Liver and Muscle in Diabetic Rats, Biomedicine and Pharmacotherapy (2019)
17. Oral Herbal Supplement Containing Magnesium Sulfate Improve Metabolic Control and Insulin Resistance in Non-Diabetic Overweight Patients: A Randomized Double Blind Clinical Trial, Medical Journal of the Islamic Republic of Iran (2019)
18. Magnesium Sulfate Improves Insulin Resistance in High Fat Diet Induced Diabetic Parents and Their Offspring, European Journal of Pharmacology (2021)
19. Insulin Resistance and the Role of Gamma-Aminobutyric Acid, Journal of Research in Medical Sciences (2021)
20. The Therapeutic Effects of Magnesium in Insulin Secretion and Insulin Resistance, Advanced Biomedical Research (2022)
21. Glp-1-Based Therapies for Type 2 Diabetes: From Single, Dual and Triple Agonists to Endogenous Glp-1 Production and L-Cell Differentiation, Diabetology and Metabolic Syndrome (2025)
22. Effect of Oral Magnesium Sulfate Administration on Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Gene Expression to Prevent Atherosclerosis in Diabetic Rat Vessels, Journal of Diabetes Investigation (2019)
23. Gaba Administration Improves Liver Function and Insulin Resistance in Offspring of Type 2 Diabetic Rats, Scientific Reports (2021)
24. Long Term Administration of Thiamine Disulfide Improves Foxo1/Pepck Pathway in Liver to Reduce Insulin Resistance in Type 1 Diabetes Rat Model, Biomedicine and Pharmacotherapy (2024)
25. The Role of Pancreas to Improve Hyperglycemia in Stz-Induced Diabetic Rats by Thiamine Disulfide, Nutrition and Diabetes (2022)
26. Possible Mechanisms and Molecular Signaling of Incretins Against the Development of Type 2 Diabetes Mellitus, Current Molecular Pharmacology (2023)
27. Effects of Salvia Mirzayanii Extract Administration on Hyperglycemia Improvement in Diabetic Rats: The Role of Glut4, Pepck and G6pase Genes, Heliyon (2024)
28. Long-Term Gaba Administration Improves Fndc5, Tfam, and Ucp3 Mrna Expressions in the Skeletal Muscle and Serum Irisin Levels in Chronic Type 2 Diabetic Rats, Naunyn-Schmiedeberg's Archives of Pharmacology (2022)