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Effect of Oral Magnesium Sulfate Administration on Blood Glucose Hemostasis Via Inhibition of Gluconeogenesis and Foxo1 Gene Expression in Liver and Muscle in Diabetic Rats Publisher Pubmed



Barooti A1 ; Kamran M1 ; Kharazmi F1 ; Eftakhar E2 ; Malekzadeh K2 ; Talebi A3 ; Soltani N4, 5
Authors
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Authors Affiliations
  1. 1. Department of Physiology, Hormozgan University of Medical Science, Bandar Abbas, Iran
  2. 2. Molecular Medicine Research Center and Hormozgan Health Institute, Hormozgan University of Medical Science, Bandar Abbas, Iran
  3. 3. Clinical Pathology Department, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran
  4. 4. Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  5. 5. Department of Physiology, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran

Source: Biomedicine and Pharmacotherapy Published:2019


Abstract

The present study was designed to investigate the possible role of Mg 2+ in suppression of phosphoenolpyruvate carboxy kinase (PEPCK) enzyme via inhibition of FOXO1gene expression in liver and we also examined whether Mg contributes to decrease blood glucose in muscle via inhibiting FOXO1 gene and protein expression. Fifty rats in five groups of experiment were considered as; non-diabetic control (NDC), Mg 2+ -treated non-diabetic control (Mg 2+ -NDC), chronic diabetic (CD), Mg 2+ -treated chronic diabetic (Mg 2+ -CD), and insulin-treated chronic diabetic (Ins-CD). Streptozotocin (STZ) was used for diabetes induction. The Mg 2+ -CD and Mg 2+ -NDC groups received 10 g/l of magnesium sulfate (MgSO 4 ) added to drinking water, and Ins-CD group received 2.5 U/kg of insulin. The blood glucose level and body weight were measured weekly. After 16 weeks, intraperitoneal glucose tolerance test (IPGTT) was done and blood samples were taken to determine the plasma levels of Mg and gastrocnemius muscle legs, and liver were isolated for both Forkhead transcription factor (FOXO1) and PEPCK enzyme genes and proteins expression. Administration of MgSO 4 improved IPGTT, lowered blood glucose levels and decreased FOXO1 and PEPCK genes and proteins expression in muscle and liver, while insulin just could decrease FOXO1 gene and protein expression in the muscle. These findings illustrated that MgSO 4 improved hyperglycemia via inhibition of FOXO1 gene and protein level in the muscle and liver, and it also decreased blood glucose level by prohibition of gluconeogenesis pathway in the liver. However, long time administration of insulin did not have any effect on liver. © 2018
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