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Improvement of Myelin Ultrastructure After Transplantation of Human Adipose Tissue-Derived Stem Cell in Rat Multiple Sclerosis Model



Ghasemi N1 ; Razavi S1 ; Salehi H1
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Authors Affiliations
  1. 1. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of MedicalSciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2016

Abstract

Background: Multiple sclerosis (MS) is a kind of the chronic neurodegenerative diseases of central nervous system (CNS) which usually is associated with neurological disability. In this study, human adipose-derived stromal/stem cells (hADSCs) were transplanted into a rat model of multiple sclerosis (MS) and the efficiency of these cells in remyelination process was determined. Methods: Forty adult rats were randomly divided into control, lysolecithin, lysolecithin with medium (vehicle), and lysolecithin with human adipose-derived stromal/stem cells transplantation groups; then, focal demyelination was induced via lysolecithin injection into lateral column of spinal cord. One week after the lysolecithin injection, laminectomy site was re-exposed and for vehicle group, 10 μl of medium and for the transplantation group 10 μl of medium containing 1 × 106 stem cells was transplanted. For the control and lysolecithin groups, just laminectomy site was re-exposed and closed again without intervention. Four weeks after the cell transplantation, immunohistochemistry technique was used for assessment of the presence of stem cells in damaged spinal cord and to assess the extent of demyelination and remyelination, transmission electron microscope was used. Findings: Immunohistochemistry study four weeks after cell transplantation showed that the stem cell transplant existed in the lesion site. In addition, the electron microscope micrographs showed that myelin synthesis increased more in the cell transplantation group compared to the other groups. Conclusion: Human adipose tissue-derived stem cell transplantation may be an appropriate method for cell therapy in neurodegenerative diseases such as multiple sclerosis. © 2016, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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