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Induction of Neuron-Like Cells From Adipose Derived Stem Cells in Alginate Hydrogel, Using Neurospheres Formation



Razavi S1 ; Khosravizadeh Z2 ; Bahramian H1 ; Kazemi M3
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Hydrogels provide appropriate three-dimensional environment for culture of a variety of cells and cell encapsulation in hydrogels is a promise plan for tissue engineering applications. Alginate is an attractive biocompatible hydrogel that provides a supportive system for the encapsulated cells. Moreover, human adipose derived stem cells are mesenchymal stem cells that might be a suitable source of cells for use in autologous cell therapy. Methods: In this study, we examined the fate of human adipose derived stem cells encapsulated in alginate hydrogel that cultured in neural induction medium for 1 week. Using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and immunocytochemical analysis, the proliferation rate, and viability and neural differentiation of human adipose derived stem cells were evaluated. Findings: We observed a significant increase in the mean percent of Nestin, glial fibrillary acidic protein (GFAP) and microtubule-associated protein-2 (MAP2) positive cells and significant reduction of proliferation rate and viability in encapsulated cells versus monolayer induced cells. Conclusion: These findings showed that alginate hydrogel can provide a suitable environment for neural differentiation of human adipose derived stem cells. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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