Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Identification of Leishmania Isolates From Healing and Nonhealing Cutaneous Leishmaniasis Patients Using Internal Transcribed Spacer Region Pcr Publisher



Tolouei S1 ; Hejazi SH1 ; Ghaedi K2 ; Hasheminia SJ3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR, Iran
  2. 2. Department of Biology, School of Sciences, University of Isfahan, Isfahan, IR, Iran
  3. 3. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR, Iran

Source: Jundishapur Journal of Microbiology Published:2014


Abstract

Background: Cutaneous Leishmaniasis (CL) is a parasitic disease in most parts of Iran, especially in the Isfahan province. The most common form of CL is a self-healing lesion but in rare situations, infection might develop to non-healing forms. Clinical symptoms and treatment process might be influenced by several agents such as host immune response and parasite strains. In this study, the isolates which caused healing and nonhealing forms of CL in Isfahan were characterized by internal transcribed spacer (ITS) polymerase chain reaction (PCR) technique. Objectives: The aim of this study was to identify Leishmania species isolated from healing and non-healing CLs using PCR method. Patients and Methods: Thirty patients resident in Isfahan province, with healing or non-healing form of CL were entered into this study. After DNA extraction, the identification of Leishmania isolates was done by ITS1-PCR method.Results: Leishmania major was found as the predominant species (100%) in both healing and non-healing forms of CL.Conclusions: It seems that there is no difference between Leishmania species in healing and non-healing forms of CL. In order to recognize the reason of long lasting lesions in non-healing patients, the study about parasite strains and immune factors at the molecular level mostly in nonhealing patient is recommended. © 2014, Ahvaz Jundishapur University of Medical Sciences.
Related Docs
View other Related Docs
1. Identification and Isolation of the Cause of Cutaneous Leishmaniasis in Isfahan Using Its-Pcr Method, Journal of Isfahan Medical School (2012)
2. Identification and Isolation of the Cause of Cutaneous Leishmaniasis in Isfahan Using Its-Pcr Method, Journal of Isfahan Medical School (2012)
3. Pcr-Rflp Diagnosis and Characterization of Leishmania Species Causing Human Cutaneous Leishmaniasis and Evaluation of Treatment Times With Glucantime in These Patients, Tropical Biomedicine (2016)
4. Molecular Epidemiological Survey of Cutaneous Leishmaniasis in Two Highly Endemic Metropolises of Iran, Application of Fta Cards for Dna Extraction From Giemsa-Stained Slides, Jundishapur Journal of Microbiology (2016)
5. Detection of Leishmania Parasites From Cutaneous Leishmaniasis Patients With Negative Direct Microscopy Using Nnn and Pcr-Rflp, Journal of Isfahan Medical School (2012)
6. Identification of Leishmania Species and Treatment Courses in Patients With Leishmaniasis in Isfahan, Iran, Journal of Isfahan Medical School (2012)
7. Genetic Variation in Rpoiils Gene Encoding Rna Polymerase Ii Largest Subunit From Leishmania Major, Molecular Biology Reports (2014)
8. The Molecular Epidemiology of Crithidia Strains in Isfahan City and Surrounding Areas, Iran, Journal of Isfahan Medical School (2014)
Experts (# of related papers)
View all Related Experts
Seyed Hossein Hejazi (57)
Ali Asilian (15)
Other Related Docs
9. Cutaneous Leishmaniasis Reinfection: Skin Disease and Leishmaniasis Research Center, Isfahan, Iran, Journal of Isfahan Medical School (2015)
10. Tlr2 and Tlr4 in Cutaneous Leishmaniasis Caused by Leishmania Major, Scandinavian Journal of Immunology (2013)
11. Comparative Molecular Epidemiology of Leishmania Major and Leishmania Tropica by Pcr-Rflp Technique in Hyper Endemic Cities of Isfahan and Bam, Iran, Case Reports and Clinical Practice Review (2010)
12. Molecular Diagnosis of Old World Leishmaniasis: Real-Time Pcr Based on Tryparedoxin Peroxidase Gene for the Detection and Identification of Leishmania Spp, Journal of Vector Borne Diseases (2012)
13. Evaluation of Il-12Rb1, Il-12B, Cxcr-3 and Il-17A Expression in Cases Affected by a Non-Healing Form of Cutaneous Leishmaniasis: An Observational Study Design, BMJ Open (2017)
14. Comparison of Three Different Therapies for Cutaneous Leishmaniasis and Identification of the Etiologic Isolates in Isfahan, Iran, Archives of Iranian Medicine (2020)
15. Comparative Molecular Epidemiology of Leishmania Major and Leishmania Tropica by Pcr-Rflp Technique in Hyper Endemic Cities of Isfahan and Bam, Iran, Medical Science Monitor (2010)
16. Genetic Analysis of Clinical Isolates of Leishmania Major From Isfahan, Iran, Journal of Vector Borne Diseases (2012)
17. Study of Il-12 Agonists in Macrophages From Patients With Healing and Non-Healing Forms of Cutaneous Leishmaniasis, Journal of Isfahan Medical School (2013)
18. Epidemiology and Clinical Features of Cutaneous Leishmaniasis in Badrood City, Iran, in 2013, Journal of Isfahan Medical School (2015)
19. Identification and Phylogenic Analysis of Leishmania Species Among Patients and Reservoir Hosts Based on N-Acetylglucosamine-1-Phosphate Transferase Gene in Central Iran, Annals of Parasitology (2022)
20. Genetic Diversity of Leishmania Major Strains Isolated From Phlebotomus Papatasi and Phlebotomus Alexandri in Western Iran Based on Minicircle Kdna, Journal of Parasitic Diseases (2021)
21. Genetic Polymorphism of Leishmania Major in Two Hyper Endemic Regions of Iran Revealed by Ppip-Pcr and Its- Rflp, Archives of Iranian Medicine (2012)
22. Population Structure of Leishmania Tropica Causing Anthroponotic Cutaneous Leishmaniasis in Southern Iran by Pcr-Rflp of Kinetoplastid Dna, BioMed Research International (2018)
23. The Epidemiology of Cutaneous Leishmaniasis in Isfahan Province, Iran, During 2001-2011, Journal of Isfahan Medical School (2015)
24. Antimonial Treatment Failure Rate in Patients With Cutaneous Leishmaniasis, Tehran University Medical Journal (2018)
25. Effect of Combination Therapy With Systemic Glucantime and Pentoxifylline in the Treatment of Cutaneous Leishmaniasis, International Journal of Dermatology (2006)
26. Serum Monocyte Chemotactic Protein-1 and Nitric Oxide in Cutaneous Leishmaniasis Patients Before and After Treatment With Glucantime, Journal of Isfahan Medical School (2012)
27. Intraspecific Variation in Leishmania Major Isolated From Different Forms of Zoonotic Cutaneous Leishmaniasis, Iranian Journal of Medical Sciences (2005)
28. Il-23 and Il-27 Levels in Macrophages Collected From Peripheral Blood of Patients With Healing Vs Non-Healing Form of Cutaneous Leishmaniasis, Iranian Journal of Parasitology (2012)
29. Molecular Identification of Leishmania Rna Virus in Cutaneous Leishmaniasis Patients and Rodent Reservoirs in Isfahan Province, Iran, Infection, Genetics and Evolution (2022)
30. A New Focus of Zoonotic Cutaneous Leishmaniasis in Shiraz, Iran, Transactions of the Royal Society of Tropical Medicine and Hygiene (2009)
31. Regulatory T-Cell Profile in Early and Late Lesions of Cutaneous Leishmaniasis Due to Leishmania Major, Journal of Research in Medical Sciences (2012)
32. Clinical Picture of Cutaneous Leishmaniasis in Isfahan, Iran, International Journal of Dermatology (1994)
33. Isolation of Bacteria Causing Secondary Bacterial Infection in the Lesions of Cutaneous Leishmaniasis, Indian Journal of Dermatology (2008)
34. Evaluation of Leishmanin Skin Test Reaction in Different Variants of Cutaneous Leishmaniasis, Indian Journal of Dermatology (2013)
35. Molecular Identification and Phylogenetic Classification of Leishmania Spp. Isolated From Human Cutaneous Leishmaniasis in Iran: A Cross-Sectional Study, Iranian Journal of Parasitology (2018)
36. Bacterial Superinfection in Zoonotic Cutaneous Leishmaniasis, Medical Science Monitor (2012)
37. An Outbreak of Cutaneous Leishmaniasis Due to Leishmania Major in an Endemic Focus in Central Iran, Journal of Parasitic Diseases (2022)
38. Serum Monocyte Chemotactic Protein-1 and Nitric Oxide in Cutaneous Leishmaniasis Patients Before and After Treatment With Glucantime, Journal of Isfahan Medical School (2012)
39. Aquaglyceroporin1 Gene Expression in Antimony Resistance and Susceptible Leishmania Major Isolates, Journal of Vector Borne Diseases (2016)
40. Regulatory T-Cell Profile in Early and Late Lesions of Cutaneous Leishmaniasis Due to Leishmania Major, Journal of Isfahan Medical School (2014)
41. Distribution Frequency of Pathogenic Bacteria Isolated From Cutaneus Leishmaniasis Lesions, Korean Journal of Parasitology (2008)
42. Population Structures of Leishmania Infantum and Leishmania Tropica the Causative Agents of Kala-Azar in Southwest Iran, Parasitology Research (2018)
43. Exposure to Leishmania Major Modulates the Proportion of Cd4+ T Cells Without Affecting Cellular Immune Responses, Microbiology and Immunology (2007)
44. Interleukin-10 and Transforming Growth Factor-Β in Early and Late Lesions of Patients With Leishmania Major Induced Cutaneous Leishmaniasis, Iranian Journal of Parasitology (2012)
45. A Comparison of the Efficiency of Molecular and Conventional Methods in the Diagnosis of Cutaneous Leishmaniosis, Annals of parasitology (2021)
46. Epidemiological Aspects of Cutaneous Leishmaniasis in Iran, Archives of Clinical Infectious Diseases (2015)
47. Behaviors and Perceptions Related to Cutaneous Leishmaniasis in Endemic Areas of the World: A Review, Acta Tropica (2021)
48. Characterization of Acid Phosphatase in the Promastigotes of Three Isolates of Leishmania Major, Iranian Journal of Medical Sciences (2003)
49. Comparison of Healing Effects of Local Injection of Glucantime With or Without Trichloroacetic Acid 50% in Cutaneous Leshmaniasis, Journal of Isfahan Medical School (2009)
50. Comparison of Protein Profile of Killed and Autoclaved Leishmania Major Using Polyacrylamide Gel Electrophoresis, Journal of Isfahan Medical School (2013)