Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Interferon-Beta-1Beta Protects Against Multiple Sclerosis-Induced Endothelial Cells Apoptosis Publisher Pubmed



Javanmard SH1 ; Mohammad Saadatnia M2, 3 ; Vida Homayouni V1 ; Mahin Nikoogoftar M4 ; Maghzi AH3, 5 ; Etemadifar M3 ; Chaitanya VG6 ; Mcgee JC7 ; Minagar A7 ; Alexander JS6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Applied Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Hezar Jerib Avenue, Iran
  2. 2. Isfahan Medical Education Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Isfahan Neurosciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Hematology, School of Medicine, Tarbiat Modarres University, Tehran, Iran
  5. 5. Neuroimmunology Unit, Centre for Neuroscience and Trauma, Blizard Institute of Cell and Molecular Science, London, United Kingdom
  6. 6. Departments of Molecular and Cellular Physiology, Shreveport, LA 71130, 1501 Kings Highway, United States
  7. 7. Departments of Neurology, Shreveport, United States

Source: Frontiers in Bioscience - Elite Published:2012


Abstract

Disruption of the blood-brain-barrier (BBB) due to endothelial cell (EC) injury is an essential step in formation of multiple sclerosis (MS) lesions. We investigated the role of endothelial cell (EC) apoptosis in the pathophysiology of MS, studying the therapeutic effect of IFN-beta-1b against MS sera-induced endothelial apoptosis. Human umbilical vein endothelial cells were treated with sera from patients with active MS (in relapse), MS in remission, or sera from healthy volunteers (each n = 5). Apoptosis was assessed by annexin V-propidium iodide staining. Effects of IFN-beta-1b on EC apoptosis were tested at increasing doses (10, 100, and1000 U/ml). Nitrite (NO2--) levels were determined in culture supernatants. EC apoptosis was increased by sera from exacerbating MS patients, but not remission, compared to healthy individuals (p<0.001). Effects were blocked by IFN-beta-1b at 10U/ml (p<0.05), but not higher doses, and was associated with increased NO/NO2- production (p<0.05). EC apoptosis leading to disruption of the BBB may play a role in MS etiology and represents a novel therapeutic mechanism of action for IFN-beta-1b in MS therapy.
Related Docs
View other Related Docs
1. Multiple Sclerosis and Cerebral Endothelial Dysfunction: Mechanisms, Pathophysiology (2011)
2. Can Vitamin D Suppress Endothelial Cells Apoptosis in Multiple Sclerosis Patients?, International Journal of Preventive Medicine (2013)
3. Multiple Sclerosis: Clinical Features, Pathophysiology, Neuroimaging and Future Therapies, Future Neurology (2009)
4. Enalapril Protects Endothelial Cells Against Induced Apoptosis in Alzheimer's Disease, Journal of Research in Medical Sciences (2013)
5. The Role of Extracellular Vesicles and Microparticles in Central Nervous System Disorders: Mechanisms, Biomarkers, and Therapeutic Potential, Cellular and Molecular Neurobiology (2024)
6. Multiple Sclerosis: Pathophysiology, Clinical Features, Diagnosis, and Management, Neuroinflammation (2011)
7. Comparison of the Expression of Mir-326 Between Interferon Beta Responders and Non-Responders in Relapsing-Remitting Multiple Sclerosis, Cell Journal (2020)
8. Mir-145 and Mir20a-5P Potentially Mediate Pleiotropic Effects of Interferon-Beta Through Mitogen-Activated Protein Kinase Signaling Pathway in Multiple Sclerosis Patients, Journal of Molecular Neuroscience (2017)
Experts (# of related papers)
View all Related Experts
Vahid Shaygannejad (6)
Masoud Etemadifar (5)
Other Related Docs
9. Therapeutic Targeting of Chemokines and Chemokine Receptors in Multiple Sclerosis: Opportunities and Challenges, CNS and Neurological Disorders - Drug Targets (2018)
10. Connection of Mir-185 and Mir-320A Expression Levels With Response to Interferon-Beta in Multiple Sclerosis Patients, Multiple Sclerosis and Related Disorders (2020)
11. Comparing Anti-Apoptotic Effects of Peroxisome Proliferator-Activated Receptor-Gamma and Angiotensin-Converting Enzyme Inhibitors Using Human Umbilical Vein Endothelial Cells Exposed to Sera From Patients With Alzheimer's Disease and Healthy Controls, Journal of Isfahan Medical School (2014)
12. Serum Homocysteine Level in Patients With Multiple Sclerosis, Journal of Research in Medical Sciences (2005)
13. Interleukin-33 Plasma Levels in Patients With Relapsing-Remitting Multiple Sclerosis, Biomolecular Concepts (2017)
14. Evaluating the Effects of Epigallocatechin-3-Gallate on Hif-1Α Protein and Rorc Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis, Basic and Clinical Neuroscience (2021)
15. The Effect of Pioglitazone on the Alzheimer’S Disease-Induced Apoptosis in Human Umbilical Vein Endothelial Cells, International Journal of Preventive Medicine (2013)
16. The Role of Micrornas Involved in the Disorder of Blood–Brain Barrier in the Pathogenesis of Multiple Sclerosis, Frontiers in Immunology (2023)
17. The Role of Nk and Nkt Cells in the Pathogenesis and Improvement of Multiple Sclerosis Following Disease-Modifying Therapies, Health Science Reports (2022)
18. Mir-504 Expression Level Is Increased in Multiple Sclerosis Patients Responder to Interferon-Beta, Journal of Neuroimmunology (2020)
19. Comparative Analysis of Apigenin-3 Acetate Versus Apigenin and Methyl-Prednisolone in Inhibiting Proliferation and Gene Expression of Th1 Cells in Multiple Sclerosis, Cell Journal (2023)
20. Significant Immunomodulatory and Hepatoprotective Impacts of Silymarin in Ms Patients: A Double-Blind Placebo-Controlled Clinical Trial, International Immunopharmacology (2021)
21. Evaluation of Matrix Metalloproteinase-9 Plasma Levels in Untreated New Relapsing–Remitting Multiple Sclerosis Patients and Their First-Degree Family, Metabolic Brain Disease (2021)
22. The Levels of Soluble Forms of Cd21 and Cd83 in Multiple Sclerosis, Journal of Neuroimmunology (2018)
23. Multiple Sclerosis: Pathogenesis, Symptoms, Diagnoses and Cell-Based Therapy, Cell Journal (2017)
24. Small Molecule of Sphingosine As a Rescue of Dopaminergic Cells: A Cell Therapy Approach in Neurodegenerative Diseases Therapeutics, Journal of Cellular Physiology (2019)
25. The Role of Cobalamin on Interleukin 10, Osteopontin, and Related Micrornas in Multiple Sclerosis, Iranian Journal of Allergy, Asthma and Immunology (2022)